The first isolation and molecular characterization of Toxoplasma gondii from horses in Serbia

被引：25

作者：

Klun, Ivana ^{[1
]}

Uzelac, Aleksandra ^{[1
]}

Villena, Isabelle ^{[2
]}

Mercier, Aurelien ^{[3
,4
]}

Bobic, Branko ^{[1
]}

Nikolic, Aleksandra ^{[1
]}

Rajnpreht, Irena ^{[1
]}

Opsteegh, Marieke ^{[5
]}

Aubert, Dominique ^{[2
]}

Blaga, Radu ^{[6
]}

van der Giessen, Joke ^{[5
]}

Djurkovic-Djakovic, Olgica ^{[1
]}

机构：

[1] Univ Belgrade, Ctr Excellence Food & Vector Borne Zoonoses, Inst Med Res, Natl Reference Lab Toxoplasmosis, Dr Subot 4, Belgrade 11129, Serbia

[2] Univ Reims, CHU Maison Blanche, Ctr Natl Reference Toxoplasmose, Lab Parasitol Mycol,EA 3800 SFR CAP SANTE,UFR Med, 45 Rue Cognacq Jay, F-51092 Reims, France

[3] Univ Limoges, INSERM, UMR S 1094, Neuroepidemiol Tropicale, 2 Rue Docteur Marcland, F-87025 Limoges, France

[4] CHU Dupuytren, Toxoplasma Biol Resource Ctr BRC, F-87042 Limoges, France

[5] Natl Inst Publ Hlth & Environm RIVM, Antonie Leeuwenhoeklaan 9, NL-3520 BA Bilthoven, Netherlands

[6] Univ Paris Est, Ecole Natl Vet Alfort, ANSES, Lab Sante Anim Maisons Alfort,UMR BIPAR, Maisons Alfort, France

来源：

PARASITES & VECTORS | 2017年 / 10卷

关键词：

Toxoplasma gondii; Horse; Serbia; Prevalence; Strain; Isolation; Genotyping; Microsatellite markers; Type III; GENETIC DIVERSITY; PREGNANT-WOMEN; INFECTION; SEROPREVALENCE; STRAINS; EQUIDS; MEAT; PIGS; CONSUMPTION; TRICHINELLA;

D O I：

10.1186/s13071-017-2104-x

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Background: Consumption of undercooked or insufficiently cured meat is a major risk factor for human infection with Toxoplasma gondii. Although horsemeat is typically consumed rare or undercooked, information on the risk of T. gondii from infected horse meat to humans is scarce. Here, we present the results of a study to determine the presence of T. gondii infection in slaughter horses in Serbia, and to attempt to isolate viable parasites. Methods: The study included horses from all regions of Serbia slaughtered at two abattoirs between June 2013 and June 2015. Blood sera were tested for the presence of specific IgG T. gondii antibodies by the modified agglutination test (MAT), and samples of trypsin-digested heart tissue were bioassayed in mice. Cyst-positive mouse brain homogenates were subjected to DNA extraction and T. gondii strains were genotyped using 15 microsatellite markers (MS). Results: A total of 105 slaughter horses were sampled. At the 1: 6 cut-off 48.6% of the examined horses were seropositive, with the highest titre being 1: 400. Viable parasites were isolated from two grade type mares; both parasite isolates (RS-Eq39 and RS-Eq40) were T. gondii type III, and both displayed an increased lethality for mice with successive passages. These are the first cases of isolation of T. gondii from horses in Serbia. When compared with a worldwide collection of 61 type III and type III-like strains, isolate RS-Eq39 showed a combination of MS lengths similar to a strain isolated from a duck in Iran, and isolate RS-Eq40 was identical in all markers to three strains isolated from a goat from Gabon, a sheep from France and a pig from Portugal. Interestingly, the source horses were one seronegative and one weakly seropositive. Conclusions: The isolation of viable T. gondii parasites from slaughter horses points to horsemeat as a potential source of human infection, but the fact that viable parasites were isolated from horses with only a serological trace of T. gondii infection presents further evidence that serology may not be adequate to assess the risk of toxoplasmosis from horsemeat consumption. Presence of T. gondii type III in Serbia sheds more light into the potential origin of this archetypal lineage in Europe.

引用

页数：9

共 55 条

[1] Genotyping of Toxoplasma gondii Isolates with 15 Microsatellite Markers in a Single Multiplex PCR Assay [J].