Minimal Residual Disease Detection by Next-Generation Sequencing in Multiple Myeloma: A Comparison With Real-Time Quantitative PCR

被引：4

作者：

Yao, Qiumei ^{[1
]}

Bai, Yinlei ^{[2
,3
]}

Kumar, Shaji ^{[4
]}

Au, Elaine ^{[5
]}

Orfao, Alberto ^{[6
,7
,8
]}

Chim, Chor Sang ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Med, Pokfulam, Queen Mary Hosp, Hong Kong, Peoples R China

[2] Tsinghua Univ, Inst Immunol, Beijing, Peoples R China

[3] Tsinghua Univ, Sch Med, Beijing, Peoples R China

[4] Mayo Clin, Div Hematol, Rochester, MN USA

[5] Univ Hong Kong, Dept Pathol, Pokfulam, Queen Mary Hosp, Hong Kong, Peoples R China

[6] Univ Salamanca USAL, Dept Med, Salamanca, Spain

[7] Univ Salamanca USAL, Cytometry Serv Nucleus, Canc Res Ctr IBMCC, USAL CSIC, Salamanca, Spain

[8] Univ Salamanca USAL, CIBERONC, Inst Biomed Res Salamanca IBSAL, Salamanca, Spain

来源：

FRONTIERS IN ONCOLOGY | 2021年 / 10卷

基金：

中国国家自然科学基金;

关键词：

minimal residual disease; multiple myeloma; next-generation sequencing; allele-specific oligonucleotide real-time quantitative-PCR; sensitivity;

D O I：

10.3389/fonc.2020.611021

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Here we compared clonotype identification by allele-specific oligonucleotide real-time quantitative-PCR (ASO RQ-PCR) and next-generation sequencing (NGS) in 80 multiple myeloma patients. ASO RQ-PCR was applicable in 49/55 (89%) and NGS in 62/78 (80%). Clonotypes identified by both methods were identical in 33/35 (94%). Sensitivity of 10(-5) was confirmed in 28/29 (96%) by NGS while sensitivity of RQ-PCR was 10(-5) in 7 (24%), 5 x 10(-5) in 15 (52%), and 10(-4) in 7 (24%). Among 14 samples quantifiable by ASO RQ-PCR, NGS yielded comparable results in 12 (86%). Applicability of NGS can be improved if immunoglobulin heavy-chain incomplete DJ primers are included.

引用

页数：5

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