Detection of coexisting bilayer gel and fluid phases by equilibrium surface pressure analysis

被引:5
作者
Buboltz, JT [1 ]
Feigenson, GW [1 ]
机构
[1] Cornell Univ, Field Biophys, Ithaca, NY 14853 USA
关键词
D O I
10.1021/la990912i
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A study of bilayer phase behavior is presented for the phospholipid mixture di12:0-PC/di16:0-PC, based on an analysis of equilibrium surface pressures which develop at the gas-water interface above suspensions of bilayer vesicles. At 22 +/- 1 degrees C, the fluidus and solidus bilayer phase boundaries are determined to be 32 and 85 mol % di16:0-PC, respectively, consistent with literature values obtained through calorimetry. Evidence is presented which suggests that Teflon substrates are better suited than high-energy substrates for surface pressure measurements involving insoluble monolayers.
引用
收藏
页码:3606 / 3611
页数:6
相关论文
共 26 条
[1]  
Bee H.E., 1983, ADSORPTION SOLUTION, P155
[2]   A STATISTICAL-THERMODYNAMIC VIEW OF COOPERATIVE STRUCTURAL-CHANGES IN PHOSPHOLIPID-BILAYER MEMBRANES - THEIR POTENTIAL ROLE IN BIOLOGICAL FUNCTION [J].
BILTONEN, RL .
JOURNAL OF CHEMICAL THERMODYNAMICS, 1990, 22 (01) :1-19
[3]   PHYSICAL-PROPERTIES OF THE FLUID LIPID-BILAYER COMPONENT OF CELL-MEMBRANES - A PERSPECTIVE [J].
BLOOM, M ;
EVANS, E ;
MOURITSEN, OG .
QUARTERLY REVIEWS OF BIOPHYSICS, 1991, 24 (03) :293-397
[4]   A novel strategy for the preparation of liposomes: rapid solvent exchange [J].
Buboltz, JT ;
Feigenson, GW .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1999, 1417 (02) :232-245
[5]  
BUBOLTZ JT, IN PRESS LANGMUIR
[6]  
CAFFREY M, 1993, LIPIDAT DATABASE THE
[7]  
EDIDIN M, 1992, COMMENTS MOL CELL BI, V8, P73
[8]   Functional roles of non-lamellar forming lipids - Preface [J].
Epand, RM .
CHEMISTRY AND PHYSICS OF LIPIDS, 1996, 81 (02) :101-104
[9]   PHYSICAL-CHEMISTRY OF LIPID FILMS AT FLUID INTERFACES [J].
GERSHFELD, NL .
ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 1976, 27 :349-368
[10]  
JONES MN, 1995, MICELLES MONOLAYERS, pCH2