Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division

被引：428

作者：

Bisson-Filho, Alexandre W. ^{[1
,2
]}

Hsu, Yen-Pang ^{[3
]}

Squyres, Georgia R. ^{[1
,2
]}

Kuru, Erkin ^{[3
,8
]}

Wu, Fabai ^{[4
,9
]}

Jukes, Calum ^{[5
]}

Sun, Yingjie ^{[1
,2
]}

Dekker, Cees ^{[4
]}

Holden, Seamus ^{[5
]}

VanNieuwenhze, Michael S. ^{[3
,6
]}

Brun, Yves V. ^{[7
]}

Garner, Ethan C. ^{[1
,2
]}

机构：

[1] Harvard Univ, Fac Arts, Mol & Cellular Biol, Cambridge, MA 02138 USA

[2] Harvard Univ, Sci Ctr Syst Biol, Cambridge, MA 02138 USA

[3] Indiana Univ, Dept Mol & Cellular Biochem, Bloomington, IN 47405 USA

[4] Delft Univ Technol, Kavli Inst Nanosci Delft, Dept Bionanosci, Delft, Netherlands

[5] Newcastle Univ, Inst Cell & Mol Biosci, Ctr Bacterial Cell Biol, Newcastle Upon Tyne NE2 4AX, Tyne & Wear, England

[6] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

[7] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA

[8] Harvard Med Sch, Dept Genet, Boston, MA 02115 USA

[9] CALTECH, Div Geol & Planetary Sci, Pasadena, CA 91125 USA

来源：

SCIENCE | 2017年 / 355卷 / 6326期

基金：

欧洲研究理事会;

关键词：

ESCHERICHIA-COLI-CELLS; D-AMINO ACIDS; ASSEMBLY DYNAMICS; IN-VIVO; BACILLUS-SUBTILIS; SUPERRESOLUTION; MICROSCOPY; MACHINERY; PROTEINS; DIVISOME;

D O I：

10.1126/science.aak9973

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The mechanism by which bacteria divide is not well understood. Cell division is mediated by filaments of FtsZ and FtsA (FtsAZ) that recruit septal peptidoglycan-synthesizing enzymes to the division site. To understand how these components coordinate to divide cells, we visualized their movements relative to the dynamics of cell wall synthesis during cytokinesis. We found that the division septum was built at discrete sites that moved around the division plane. FtsAZ filaments treadmilled circumferentially around the division ring and drove the motions of the peptidoglycan-synthesizing enzymes. The FtsZ treadmilling rate controlled both the rate of peptidoglycan synthesis and cell division. Thus, FtsZ treadmilling guides the progressive insertion of new cell wall by building increasingly smaller concentric rings of peptidoglycan to divide the cell.

引用

页码：739 / 743

页数：5

共 24 条

[1] Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins [J].

Anderson, DE ;

Gueiros-Filho, FJ ;

Erickson, HP .

JOURNAL OF BACTERIOLOGY, 2004, 186 (17) :5775-5781

[2] FtsZ filament capping by MciZ, a developmental regulator of bacterial division [J].

Bisson-Filho, Alexandre W. ;

Discola, Karen F. ;

Castellen, Patricia ;

Blasios, Valdir ;

Martins, Alexandre ;

Sforca, Mauricio L. ;

Garcia, Wanius ;

Zeri, Ana Carolina M. ;

Erickson, Harold P. ;

Dessen, Andrea ;

Gueiros-Filho, Frederico J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (17) :E2130-E2138

[3] Rapid in vitro assembly dynamics and subunit turnover of FtsZ demonstrated by fluorescence resonance energy transfer [J].

Chen, YD ;

Erickson, HP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (23) :22549-22554

[4] Defining the rate-limiting processes of bacterial cytokinesis [J].

Coltharp, Carla ;

Buss, Jackson ;

Plumer, Trevor M. ;

Xiao, Jie .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (08) :E1044-E1053

[5] Probing the catalytic activity of a cell division-specific transpeptidase in vivo with β-lactams [J].

Eberhardt, C ;

Kuerschner, L ;

Weiss, DS .

JOURNAL OF BACTERIOLOGY, 2003, 185 (13) :3726-3734

[6] The stoichiometric divisome: a hypothesis [J].

Egan, Alexander J. F. ;

Vollmer, Waldemar .

FRONTIERS IN MICROBIOLOGY, 2015, 6

[7] Two-Step Assembly Dynamics of the Bacillus subtilis Divisome [J].

Gamba, Pamela ;

Veening, Jan-Willem ;

Saunders, Nigel J. ;

Hamoen, Leendert W. ;

Daniel, Richard A. .

JOURNAL OF BACTERIOLOGY, 2009, 191 (13) :4186-4194

[8] Coupled, Circumferential Motions of the Cell Wall Synthesis Machinery and MreB Filaments in B. subtilis [J].

Garner, Ethan C. ;

Bernard, Remi ;

Wang, Wenqin ;

Zhuang, Xiaowei ;

Rudner, David Z. ;

Mitchison, Tim .

SCIENCE, 2011, 333 (6039) :222-225

[9]

Grimm JB, 2015, NAT METHODS, V12, P244, DOI [10.1038/NMETH.3256, 10.1038/nmeth.3256]

[10] An inhibitor of FtsZ with potent and selective anti-staphylococcal activity [J].

Haydon, David J. ;

Stokes, Neil R. ;

Ure, Rebecca ;

Galbraith, Greta ;

Bennett, James M. ;

Brown, David R. ;

Baker, Patrick J. ;

Barynin, Vladimir V. ;

Rice, David W. ;

Sedelnikova, Sveta E. ;

Heal, Jonathan R. ;

Sheridan, Joseph M. ;

Aiwale, Sachin T. ;

Chauhan, Pramod K. ;

Srivastava, Anil ;

Taneja, Amit ;

Collins, Ian ;

Errington, Jeff ;

Czaplewski, Lloyd G. .

SCIENCE, 2008, 321 (5896) :1673-1675

← 1 2 3 →