A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

被引：19

作者：

Adams, La'Tonzia L. ^{[1
]}

Salee, Parichat ^{[1
,2
]}

Dionne, Kim ^{[1
]}

Carroll, Karen ^{[1
]}

Parrish, Nicole ^{[1
]}

机构：

[1] Johns Hopkins Med Inst, Baltimore, MD USA

[2] Chiang Mai Univ, Chiang Mai 50000, Thailand

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 119卷

关键词：

Mycobacterium; Protein extraction; MALDI-ToF MS; DESORPTION IONIZATION-TIME; SOLID-CULTURE-MEDIA; RAPID IDENTIFICATION; MASS-SPECTROMETRY; INACTIVATION;

D O I：

10.1016/j.mimet.2015.09.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard. (C) 2015 Elsevier B.V. All rights reserved.

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页码：1 / 3

页数：3

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