A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

被引：19
作者：
Adams, La'Tonzia L. [1 ]
Salee, Parichat [1 ,2 ]
Dionne, Kim [1 ]
Carroll, Karen [1 ]
Parrish, Nicole [1 ]
机构：
[1] Johns Hopkins Med Inst, Baltimore, MD USA
[2] Chiang Mai Univ, Chiang Mai 50000, Thailand
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2015年 / 119卷
关键词：
Mycobacterium; Protein extraction; MALDI-ToF MS; DESORPTION IONIZATION-TIME; SOLID-CULTURE-MEDIA; RAPID IDENTIFICATION; MASS-SPECTROMETRY; INACTIVATION;
D O I：
10.1016/j.mimet.2015.09.010
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：1 / 3
页数：3
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