Benzoate Catabolite Repression of the Phenol Degradation in Acinetobacter calcoaceticus PHEA-2

被引：21

作者：

Zhan, Yuhua ^{[1
,2
]}

Yu, Haiying ^{[1
,2
]}

Yan, Yongliang ^{[2
]}

Ping, Shuzhen ^{[2
]}

Lu, Wei ^{[2
]}

Zhang, Wei ^{[2
]}

Chen, Ming ^{[2
]}

Lin, Min ^{[1
,2
]}

机构：

[1] China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China

[2] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

来源：

CURRENT MICROBIOLOGY | 2009年 / 59卷 / 04期

基金：

中国国家自然科学基金;

关键词：

PSEUDOMONAS-PUTIDA; AROMATIC-COMPOUNDS; GENE-EXPRESSION; PATHWAY; BACTERIA; INHIBITION; REGULATORS;

D O I：

10.1007/s00284-009-9446-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Acinetobacter calcoaceticus PHEA-2 exhibited a delayed utilization of phenol in the presence of benzoate. Benzoate supplementation completely inhibited phenol degradation in a benzoate 1,2-dioxygenase knockout mutant. The mphR encoding the transcriptional activator and mphN encoding the largest subunit of multi-component phenol hydroxylase in the benA mutant were significantly downregulated (about 7- and 70-fold) on the basis of mRNA levels when benzoate was added to the medium. The co-transformant assay of E. coli JM109 with mphK::lacZ fusion and the plasmid pETR carrying mphR gene showed that MphR did not activate the mph promoter in the presence of benzoate. These results suggest that catabolite repression of phenol degradation by benzoate in A. calcoaceticus PHEA-2 is mediated by the inhibition of the activator protein MphR.

引用

页码：368 / 373

页数：6

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