Reduction of nitro phenols using nitroreductase from E-coli in the presence of NADH

被引：96

作者：

Dai, Rongji ^{[1
]}

Chen, Jie ^{[1
,2
]}

Lin, Jie ^{[1
]}

Xiao, Shenyuan ^{[2
]}

Chen, Shi ^{[1
]}

Deng, Yulin ^{[1
]}

机构：

[1] Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China

[2] Beijing Univ Chinese Med, Dongfang Coll, Langfang 065001, Hebei, Peoples R China

来源：

JOURNAL OF HAZARDOUS MATERIALS | 2009年 / 170卷 / 01期

关键词：

Nitroreductase; Nitrophenols; Reduction; CATALYZED BIOTRANSFORMATION; MOLECULAR-STRUCTURE; P-NITROPHENOL; PICRIC ACID; DEGRADATION; COMPLEXES; 2,4-DINITROPHENOL; 4-NITROPHENOL; 2,4,6-TRINITROTOLUENE; BIODEGRADATION;

D O I：

10.1016/j.jhazmat.2009.04.122

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The reductions of nitrophenols catalyzed by nitroreductase from E. coli in the presence of NADH were investigated in this paper. 4-Aminophenol and 4-hydroxylaminophenol were found in the reductive products of 4-nitrophenol and the maximum reductive ratio was about 83.49% when the reaction time was 70 min; 4,6-dinitro-2-pimelie kelone was found in the reductive products of 2,4-dinitrophenol and the maximum reductive ratio was about 75.28% when the reaction time was 80 min; 2,4-dinitrophenol and 4,6-dinitro-2-pimelie kelone were found in the reductive products of 2,4,6-trinitrophenol and the maximum reductive ratio was about 62.08% when the reaction time was 100 min. The similar reductive ratios of nitrophenols were obtained under aerobic and anaerobic conditions. The results indicated that nitroreductase was an oxygen-insensitive enzyme. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：141 / 143

页数：3

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