A highly selective fluorescent probe for sensitive detection of HAV in water

被引：6

作者：

Xie, Jun ^{[1
]}

Zhou, Peng ^{[1
]}

Wen, Min ^{[1
]}

Bai, Jingyu ^{[1
]}

Yang, Fangming ^{[1
]}

Duan, Chunying ^{[2
]}

机构：

[1] Dalian Univ Technol, Coll Chem, Dalian 116024, Peoples R China

[2] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

来源：

ANALYTICAL METHODS | 2019年 / 11卷 / 26期

基金：

中国国家自然科学基金;

关键词：

HEPATITIS-A-VIRUS; RESONANCE ENERGY-TRANSFER; CDTE QUANTUM DOTS; DNA-SYNTHESIS; FRET; QUANTIFICATION; AMPLIFICATION; NANOSENSOR; NOROVIRUS; TARGET;

D O I：

10.1039/c9ay00831d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Highly specific and sensitive detection of hepatitis A virus (HAV) in water is of great significance for drinking water safety and epidemic control. In this work, a hybrid nanobiosensor for HAV detection was established based on fluorescence resonance energy transfer (FRET) between quantum dots (QDs) as a donor and Cy5 as an acceptor. Transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and dynamic light scattering were used to characterize the chemical and physical structures of the hybrid biosensor. Under optimized conditions, the detection limit of target DNA (tDNA) was 1.3 x 10(-11) mol L-1 (S/N = 3), with a linear range of 2 x 10(-10) mol L-1 to 5 x 10(-8) mol L-1. In addition, it showed excellent selectivity in the presence of various interfering species. The results show the applicability of the biosensor for monitoring HAV in environmental and food samples.

引用

页码：3350 / 3357

页数：8

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