A highly selective fluorescent probe for sensitive detection of HAV in water

被引：6

作者：

Xie, Jun ^{[1
]}

Zhou, Peng ^{[1
]}

Wen, Min ^{[1
]}

Bai, Jingyu ^{[1
]}

Yang, Fangming ^{[1
]}

Duan, Chunying ^{[2
]}

机构：

[1] Dalian Univ Technol, Coll Chem, Dalian 116024, Peoples R China

[2] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

来源：

ANALYTICAL METHODS | 2019年 / 11卷 / 26期

基金：

中国国家自然科学基金;

关键词：

HEPATITIS-A-VIRUS; RESONANCE ENERGY-TRANSFER; CDTE QUANTUM DOTS; DNA-SYNTHESIS; FRET; QUANTIFICATION; AMPLIFICATION; NANOSENSOR; NOROVIRUS; TARGET;

D O I：

10.1039/c9ay00831d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Highly specific and sensitive detection of hepatitis A virus (HAV) in water is of great significance for drinking water safety and epidemic control. In this work, a hybrid nanobiosensor for HAV detection was established based on fluorescence resonance energy transfer (FRET) between quantum dots (QDs) as a donor and Cy5 as an acceptor. Transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy and dynamic light scattering were used to characterize the chemical and physical structures of the hybrid biosensor. Under optimized conditions, the detection limit of target DNA (tDNA) was 1.3 x 10(-11) mol L-1 (S/N = 3), with a linear range of 2 x 10(-10) mol L-1 to 5 x 10(-8) mol L-1. In addition, it showed excellent selectivity in the presence of various interfering species. The results show the applicability of the biosensor for monitoring HAV in environmental and food samples.

引用

页码：3350 / 3357

页数：8

共 45 条

[1]

Akihiko H., 2011, APPL ENVIRON MICROB, V77, P4336

[2] Fluorescence lifetime imaging by time-correlated single-photon counting [J].

Becker, W ;

Bergmann, A ;

Hink, MA ;

König, K ;

Benndorf, K ;

Biskup, C .

MICROSCOPY RESEARCH AND TECHNIQUE, 2004, 63 (01) :58-66

[3] Seroprevalence and susceptibility to hepatitis A in the European Union and European Economic Area: a systematic review [J].

Carrillo-Santisteve, Paloma ;

Tavoschi, Lara ;

Severi, Ettore ;

Bonfigli, Sandro ;

Edelstein, Michael ;

Bystrom, Emma ;

Lopalco, Pierluigi .

LANCET INFECTIOUS DISEASES, 2017, 17 (10) :E306-E319

[4] Outbreak of hepatitis A in the USA associated with frozen pomegranate arils imported from Turkey: an epidemiological case study [J].

Collier, Melissa G. ;

Khudyakov, Yury E. ;

Selvage, David ;

Adams-Cameron, Meg ;

Epson, Erin ;

Cronquist, Alicia ;

Jervis, Rachel H. ;

Lamba, Katherine ;

Kimura, Akiko C. ;

Sowadsky, Rick ;

Hassan, Rashida ;

Park, Sarah Y. ;

Garza, Eric ;

Elliott, Aleisha J. ;

Rotstein, David S. ;

Beal, Jennifer ;

Kuntz, Thomas ;

Lance, Susan E. ;

Dreisch, Rebecca ;

Wise, Matthew E. ;

Nelson, Noele P. ;

Suryaprasad, Anil ;

Drobeniuc, Jan ;

Holmberg, Scott D. ;

Xu, Fujie .

LANCET INFECTIOUS DISEASES, 2014, 14 (10) :976-981

[5] A comparative study of digital RT-PCR and RT-qPCR for quantification of Hepatitis A virus and Norovirus in lettuce and water samples [J].

Coudray-Meunier, Coralie ;

Fraisse, Audrey ;

Martin-Latil, Sandra ;

Guillier, Laurent ;

Delannoy, Sabine ;

Fach, Patrick ;

Perelle, Sylvie .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2015, 201 :17-26

[6] Genetic variability and molecular evolution of Hepatitis A virus [J].

Cristina, Juan ;

Costa-Mattioli, Mauro .

VIRUS RESEARCH, 2007, 127 (02) :151-157

[7] Hepatitis A virus in environmental water samples from the Amazon Basin [J].

De Paula, V. S. ;

Diniz-Mendes, L. ;

Villar, L. M. ;

Luz, S. L. B. ;

Silva, L. A. ;

Jesus, M. S. ;

da Silva, N. M. V. S. ;

Gaspar, A. M. C. .

WATER RESEARCH, 2007, 41 (06) :1169-1176

[8] CdTe QDs-based prostate-specific antigen probe for human prostate cancer cell imaging [J].

Dong, Wei ;

Guo, Li ;

Wang, Meng ;

Xu, Shukun .

JOURNAL OF LUMINESCENCE, 2009, 129 (09) :926-930

[9] FRET two-hybrid mapping reveals function and location of L-type Ca2+ channel CaM preassociation [J].

Erickson, MG ;

Liang, HY ;

Mori, MX ;

Yue, DT .

NEURON, 2003, 39 (01) :97-107

[10] A single-molecule FRET sensor for monitoring DNA synthesis in real time [J].

Fijen, Carel ;

Silva, Alejandro Monton ;

Hochkoeppler, Alejandro ;

Hohlbein, Johannes .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2017, 19 (06) :4222-4230

← 1 2 3 4 5 →