Detection and genotyping of flavobacterium psychrophilum by PCR targeted to peptidyl-prolyl cis-trans isomerase C gene

被引：27

作者：

Yoshiura, Yasutoshi ^{[1
]}

Kamaishi, Takashi

Nakayasu, Chihaya

Ototake, Mitsuru

机构：

[1] Natl Res Inst Aquaculture, Fisheries Res Agcy, Aquat Anim Hlth Div, Tamaki, Mie 5190423, Japan

[2] Natl Res Inst Aquaculture, Fisheries Res Agcy, Aquat Anim Hlth Div, Minami Ise, Mie 5160193, Japan

来源：

FISH PATHOLOGY | 2006年 / 41卷 / 02期

关键词：

Flavobacterium psychrophilum; Plecoglossus altivelis; ayu; peptidyl-prolyl cis-trans isomerase C (PPIC); genotype; coldwater disease;

D O I：

10.3147/jsfp.41.67

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Flavobacterium psychrophilum can be classified into two genotypes, A and B, by the polymorphism in an unexpected product of 290 bp that is amplified with universal primers for gyrase subunit B gene. We identified the 290 bp fragment as a part of coding region of peptidyl-prolyl cistrans isomerase C (PPIC) gene. Using PCR newly designed for PPIC gene, specific amplification was observed in all F psychrophilum strains tested, and no amplification occurred in any other bacterial species tested. Moreover, genotype A was found only in isolates from ayu Plecoglossus altivelis.

引用

页码：67 / 71

页数：5

共 4 条

[1] PEPTIDYLPROLINE CIS/TRANS ISOMERASES [J].