The Role of the 145 Residue in Photochemical Properties of the Biphotochromic Protein mSAASoti: Brightness versus Photoconversion

被引：0

作者：

Gavshina, Alexandra V. ^{[1
]}

Solovyev, Ilya D. ^{[1
]}

Savitsky, Alexander P. ^{[1
]}

机构：

[1] Russian Acad Sci, AN Bach Inst Biochem, Fed Res Ctr Fundamentals Biotechnol, Leninsky Ave 33,Bld 2, Moscow 119071, Russia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 24期

关键词：

biphotochromic fluorescent proteins; brightness; photoconversion green to red; photoswitching; photochemistry of fluorescent proteins; SWITCHABLE FLUORESCENT PROTEINS; LIVE-CELL; MICROSCOPY;

D O I：

10.3390/ijms232416058

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Photoswitchable fluorescent proteins (FPs) have become indispensable tools for studying life sciences. mSAASoti FP, a biphotochromic FP, is an important representative of this protein family. We created a series of mSAASoti mutants in order to obtain fast photoswitchable variants with high brightness. K145P mSAASoti has the highest molar extinction coefficient of all SAASoti mutants studied; C21N/K145P/M163A switches to the dark state 36 times faster than mSAASoti, but it lost its ability to undergo green-to-red photoconversion. Finally, the C21N/K145P/F177S and C21N/K145P/M163A/F177S variants demonstrated a high photoswitching rate between both green and red forms.

引用

页数：9

共 28 条

[1] Rational Design of Photoconvertible and Biphotochromic Fluorescent Proteins for Advanced Microscopy Applications [J].

Adam, Virgile ;

Moeyaert, Benjamien ;

David, Charlotte C. ;

Mizuno, Hideaki ;

Lelimousin, Mickael ;

Dedecker, Peter ;

Ando, Ryoko ;

Miyawaki, Atsushi ;

Michiels, Jan ;

Engelborghs, Yves ;

Hofkens, Johan .

CHEMISTRY & BIOLOGY, 2011, 18 (10) :1241-1251

[2] Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations [J].

Adam, Virgile ;

Lelimousin, Mickal ;

Boehme, Susan ;

Desfonds, Guillaume ;

Nienhaus, Karin ;

Field, Martin J. ;

Wiedenmann, Joerg ;

McSweeney, Sean ;

Nienhaus, G. Ulrich ;

Bourgeois, Dominique .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (47) :18343-18348

[3] Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy [J].

Andresen, Martin ;

Stiel, Andre C. ;

Foelling, Jonas ;

Wenzel, Dirk ;

Schoenle, Andreas ;

Egner, Alexander ;

Eggeling, Christian ;

Hell, Stefan W. ;

Jakobs, Stefan .

NATURE BIOTECHNOLOGY, 2008, 26 (09) :1035-1040

[4] Structural basis for reversible photoswitching in Dronpa [J].

Andresen, Martin ;

Stiel, Andre C. ;

Trowitzsch, Simon ;

Weber, Gert ;

Eggeling, Christian ;

Wahl, Markus C. ;

Hell, Stefan W. ;

Jakobs, Stefan .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (32) :13005-13009

[5] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[6] Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability [J].

Bossi, Mariano ;

Foelling, Jonas ;

Dyba, Marcus ;

Westphal, Volker ;

Hell, Stefan W. .

NEW JOURNAL OF PHYSICS, 2006, 8

[7] Molecular Basis of the Light-driven Switching of the Photochromic Fluorescent Protein Padron [J].

Brakemann, Tanja ;

Weber, Gert ;

Andresen, Martin ;

Groenhof, Gerrit ;

Stiel, Andre C. ;

Trowitzsch, Simon ;

Eggeling, Christian ;

Grubmueller, Helmut ;

Hell, Stefan W. ;

Wahl, Markus C. ;

Jakobs, Stefan .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (19) :14603-14609

[8] A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications [J].

Chang, Hao ;

Zhang, Mingshu ;

Ji, Wei ;

Chen, Juanjuan ;

Zhang, Yongdeng ;

Liu, Bei ;

Lu, Jingze ;

Zhang, Junlong ;

Xu, Pingyong ;

Xu, Tao .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (12) :4455-4460

[9] Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins [J].

Chang, Jeffrey ;

Romei, Matthew G. ;

Boxer, Steven G. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2019, 141 (39) :15504-15508

[10] Widely accessible method for superresolution fluorescence imaging of living systems [J].

Dedecker, Peter ;

Mo, Gary C. H. ;

Dertinger, Thomas ;

Zhang, Jin .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (27) :10909-10914

← 1 2 3 →