Determination of the residues of 50 anabolic hormones in muscle, milk and liver by very-high-pressure liquid chromatography-electrospray ionization tandem mass spectrometry

被引：101

作者：

Yang, Yi ^{[1
]}

Shao, Bing ^{[1
,2
]}

Zhang, Jing ^{[1
]}

Wu, Yongning ^{[1
,2
]}

Duan, Hejun ^{[1
]}

机构：

[1] Beijing Ctr Dis Prevent & Control, Cent Lab, Beijing 100013, Peoples R China

[2] Capital Med Univ, Sch Publ Hlth & Family Med, Beijing 100089, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 5-6期

关键词：

Anabolic hormones; LC-ESI-MS/MS; Foods of animal origin; Solid-phase extraction; CHEMICAL-IONIZATION; SAMPLE PREPARATION; STEROID-HORMONES; EXTRACTION; TISSUES; URINE; MEAT; CONTRACEPTIVES; VALIDATION; ANDROGENS;

D O I：

10.1016/j.jchromb.2008.12.054

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and specific method for determination of the residues of 50 anabolic hormones in muscle (pork, beef, shrimp), milk and pig liver was developed. Analytes were separated and acquired by liquid chromatography coupled with an electrospray ionization tandem mass spectrometer (LC-ESI-MS/MS). Target compounds were simultaneously extracted with methanol after enzyme hydrolysis, and purified using a graphitized carbon-black solid-phase extraction (SPE) and followed by NH2 SPE cartridge. Limits of quantification were 0.04-2.0 mu g kg (1); average recoveries were 76.9-121.3%; and the relative standard deviation was 2.4-21.2%. This method has been successfully applied in real samples. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：489 / 496

页数：8

共 34 条

[1]

ANGERSSON AM, 1999, J ENDOCRINOL, V140, P477

[2]

[Anonymous], 1996, P EUR WORKSH IMP END

[3] The challenge posed by endocrine-disrupting chemicals [J].

Ashby, J ;

Houthoff, E ;

Kennedy, SJ ;

Stevens, J ;

Bars, R ;

Jekat, FW ;

Campbell, P ;

VanMiller, J ;

Carpanini, FM ;

Randall, GLP .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1997, 105 (02) :164-169

[4] Analysis of meat samples for anabolic steroids residues by liquid chromatography/tandem mass spectrometry [J].

Blasco, Cristina ;

Van Poucke, Christof ;

Van Peteghem, Carlos .

JOURNAL OF CHROMATOGRAPHY A, 2007, 1154 (1-2) :230-239

[5]

BRABANDER HFD, 1996, J CHROMATOGR A, V750, P105

[6] Solid phase extraction of trace elements [J].

Camel, V .

SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, 2003, 58 (07) :1177-1233

[7] Quantitative determination of dexamethasone in bovine plasma and tissues by liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry to monitor residue depletion kinetics [J].

Cherlet, M ;

De Baere, S ;

Croubels, S ;

De Backer, P .

ANALYTICA CHIMICA ACTA, 2005, 529 (1-2) :361-369

[8]

*COMM EUR COMM, 9622EC

[9] Determination of naturally occurring oestrogens and androgens in retail samples of milk and eggs [J].

Courant, F. ;

Antignac, J. -P. ;

Maume, D. ;

Monteau, F. ;

Andre, F. ;

Le Bizec, B. .

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2007, 24 (12) :1358-1366

[10] Validation of multi-residue methods for the detection of anabolic steroids by GC-MS in muscle tissues and urine samples from cattle [J].

Daeseleire, E ;

Vandeputte, R ;

Van Peteghem, C .

ANALYST, 1998, 123 (12) :2595-2598

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