Evaluation of real-time PCR for quantitative detection of Escherichia coli in beach water

被引：9

作者：

Lam, Jason Tszhin ^{[1
]}

Lui, Edwin ^{[2
]}

Chau, Simon ^{[2
]}

Kueh, Cathie Show Wu ^{[3
]}

Yung, Ying-kit ^{[3
]}

Yam, Wing Cheong ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Microbiol, Queen Mary Hosp Compound, Hong Kong, Hong Kong, Peoples R China

[2] Hong Kong Govt Environm Protect Dept, Environm Compliance Div, Wan Chai, Hong Kong, Peoples R China

[3] Hong Kong Govt Environm Protect Dept, Water Policy & Planning Div, Wan Chai, Hong Kong, Peoples R China

来源：

JOURNAL OF WATER AND HEALTH | 2014年 / 12卷 / 01期

关键词：

cyd; Escherichia coli; hybridization probes; marine beach water; real-time PCR; POLYMERASE-CHAIN-REACTION; HONG-KONG; ENUMERATION; POLLUTION; QUALITY;

D O I：

10.2166/wh.2013.038

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The current investigation evaluated the use of real-time polymerase chain reaction (PCR) for quantitative detection of Escherichia coli in marine beach water. Densities of E. coli in 263 beach water samples collected from 13 bathing beaches in Hong Kong between November 2008 and December 2009 were determined using both real-time PCR and culture-based methods. Regression analysis showed that these two methods had a significant positive linear relationship with a correlation coefficient (r) of 0.64. Serial dilution of spiked samples indicated that the real-time PCR had a limit of quantification of 25 E. coli colonies in 100 mL water sample. This study showed that the rapid real-time PCR has potential to complement the traditional culture method of assessing fecal pollution in marine beach water.

引用

页码：51 / 56

页数：6

共 12 条

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