Type V collagen counteracts osteo-differentiation of human mesenchymal stem cells

被引：13

作者：

Longo, Alessandra ^{[1
]}

Tobiasch, Edda ^{[2
]}

Luparello, Claudio ^{[1
]}

机构：

[1] Univ Palermo, Dipartimento Sci Tecnol Biol Chim & Farmaceut STE, I-90128 Palermo, Italy

[2] Bonn Rhein Sieg Univ, Dept Nat Sci, Rheinbach, Germany

来源：

BIOLOGICALS | 2014年 / 42卷 / 05期

关键词：

Stem cells; Collagen; Osteogenesis; Gene expression; BREAST-CANCER CELLS; CARCINOMA CELLS; EXPRESSION; ADHESION; BONE; MIGRATION; MOTILITY; BEHAVIOR; STRESS; FIBRIL;

D O I：

10.1016/j.biologicals.2014.07.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In search of novel gene signatures for osteo-differentiation of mesenchymal stem cells (MSCs), we submitted cDNA preparations from undifferentiated and differentiating MSCs to differential display- and semiquantitative-PCR and found down-regulation of COL5A1 in osteo-induced cultures at days 21 and 28, when the mineralized matrix accumulates. We also cultured osteo-differentiating MSCs onto type V collagen substrates and found a decrease in the accumulation of extracellular calcium compared to those grown in uncoated flasks. To our knowledge, this is first evidence that type V collagen might represent a stromal component that impairs osteogenesis. (C) 2014 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.

引用

收藏

页码：294 / 297

页数：4

相关论文

共 23 条

[1] Type V collagen: heterotypic type I/V collagen interactions in the regulation of fibril assembly [J].

Birk, DE .

MICRON, 2001, 32 (03) :223-237

[2] Collagen type V modulates fibroblast behavior dependent on substrate stiffness [J].

Breuls, Roel G. M. ;

Klumpers, Darinka D. ;

Everts, Vincent ;

Smit, Theo H. .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 380 (02) :425-429

[3] Schwann cell type v collagen inhibits axonal outgrowth and promotes Schwann cell migration via distinct adhesive activities of the collagen and noncollagen domains [J].

Chernousov, MA ;

Stahl, RC ;

Carey, DJ .

JOURNAL OF NEUROSCIENCE, 2001, 21 (16) :6125-6135

[4] Isolation and characterization of multipotent postnatal stem/progenitor cells from human alveolar bone proper [J].

El-Sayed, Karim M. Fawzy ;

Paris, Sebastian ;

Becker, Stephan ;

Kassem, Neemat ;

Ungefroren, Hendrick ;

Faendrich, Fred ;

Wiltfang, Joerg ;

Doerfer, Christof .

JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY, 2012, 40 (08) :735-742

[5] Collagens -: structure, function, and biosynthesis [J].

Gelse, K ;

Pöschl, E ;

Aigner, T .

ADVANCED DRUG DELIVERY REVIEWS, 2003, 55 (12) :1531-1546

[6] THE STRO-1(+) FRACTION OF ADULT HUMAN BONE-MARROW CONTAINS THE OSTEOGENIC PRECURSORS [J].

GRONTHOS, S ;

GRAVES, SE ;

OHTA, S ;

SIMMONS, PJ .

BLOOD, 1994, 84 (12) :4164-4173

[7]

Khan Wasim S, 2012, J Stem Cells, V7, P75

[8] Influence of various cytokines on adhesion and migration of the colorectal adenoarcinoma cell line HRT-18 [J].

Köbel, M ;

Budianto, D ;

Schmitt, WD ;

Borsi, L ;

Siri, A ;

Hauptmann, S .

ONCOLOGY, 2005, 68 (01) :33-39

[9] PTHrP in differentiating human mesenchymal stem cells: Transcript isoform expression, promoter methylation, and protein accumulation [J].

Longo, Alessandra ;

Librizzi, Mariangela ;

Naselli, Flores ;

Caradonna, Fabio ;

Tobiasch, Edda ;

Luparello, Claudio .

BIOCHIMIE, 2013, 95 (10) :1888-1896

[10] Effect of transfection with PLP2 antisense oligonucleotides on gene expression of cadmium-treated MDA-MB231 breast cancer cells [J].

Longo, Alessandra ;

Librizzi, Mariangela ;

Luparello, Claudio .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2013, 405 (06) :1893-1901