Determination of Amino Acids in Corn Seed by Gas Chromatography-Mass Spectrometry

被引：16

作者：

Culea, Monica ^{[1
]}

Scrob, Stancuta ^{[2
]}

Suvar, Sonia ^{[1
]}

Podea, Paula ^{[3
]}

Has, Ioan ^{[2
]}

Muste, Sevastita ^{[2
]}

机构：

[1] Univ Babes Bolyai, Fac Phys, R-3400 Cluj Napoca, Romania

[2] Univ Agr Sci & Vet Med, Fac Food Sci & Technol, Cluj Napoca, Romania

[3] Univ Babes Bolyai, Fac Chem & Chem Engn, R-3400 Cluj Napoca, Romania

来源：

ANALYTICAL LETTERS | 2015年 / 48卷 / 01期

关键词：

Amino acids; Corn; Inbred lines; GC/MS; QUALITY PROTEIN MAIZE; GC-MS; BIOLOGICAL MEDIA; GRAIN; ENVIRONMENT; ENDOSPERM; GENETICS;

D O I：

10.1080/00032719.2014.930869

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A gas chromatography-mass spectrometry method was developed for the determination of free amino acids from corn inbred lines flour. Amino acids were extracted using 6% trichloroacetic acid. The extracts were purified using an ion-exchange solid phase, derivatized as trifluoroacetic butyl esters, and analyzed. The N-15-methionine was used as the internal standard. The method was repeatable for most of the amino acids (coefficient of variation was in the order of 20%), and good accuracy and limits of detection were obtained. Among the free amino acids determined, the highest concentrations were obtained for aspartic acid, proline, gamma-aminobutyric acid, lysine, alanine, glutamic acid, and histidine. The study established the variation of the free amino acids within the inbred lines.

引用

页码：37 / 46

页数：10

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