Tools for Studying Glycans: Recent Advances in Chemoenzymatic Glycan Labeling

被引：90

作者：

Aguilar, Aime Lopez ^{[1
]}

Briard, Jennie Grace ^{[1
]}

Yang, Linette ^{[2
]}

Ovryn, Ben ^{[1
]}

Macauley, Matthew Scott ^{[1
]}

Wu, Peng ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Physiol Chem, La Jolla, CA 92037 USA

[2] Vassar Coll, 124 Raymond Ave, Poughkeepsie, NY 12604 USA

来源：

ACS CHEMICAL BIOLOGY | 2017年 / 12卷 / 03期

基金：

美国国家卫生研究院;

关键词：

GLCNAC-MODIFIED PROTEINS; O-LINKED GLYCOSYLATION; CELL-SURFACE GLYCANS; FREE CLICK CHEMISTRY; SIALIC-ACID; LIVING CELLS; COPPER-FREE; IN-VIVO; SUBSTRATE FLEXIBILITY; N-ACETYLLACTOSAMINE;

D O I：

10.1021/acschembio.6b01089

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The study of cellular glycosylation presents many challenges due, in large part, to the nontemplated nature of glycan biosynthesis and glycans' structural complexity. Chemoenzymatic glycan labeling (CeGL) has emerged as a new technique to address the limitations of existing methods for glycan detection. CeGL combines glycosyltransferases and unnatural nudeotide sugar donors equipped with a bioorthogonal chemical tag to directly label specific glycan acceptor substrates in situ within biological samples. This article reviews the current CeGL strategies that are available to characterize cell-surface and intracellular glycans. Applications include imaging glycan expression status in live cells and tissue samples, proteomic analysis of glycoproteins, and target validation. Combined with genetic and biochemical tools, CeGL provides new opportunities to elucidate the functional roles of glycans in human health and disease.

引用

页码：611 / 621

页数：11

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