Characterization of oligonucleotide aptamers targeting the 5′-UTR from dengue virus

被引：8

作者：

Cnossen, Elismar J. N. ^{[1
,2
]}

Silva, Amanda G. ^{[1
,2
]}

Marangoni, Karina ^{[3
]}

Arruda, Rivia A. R. ^{[1
,2
]}

Souza, Evellyn G. ^{[1
,2
]}

Santos, Fabiana A. A. ^{[3
]}

Fujimura, Patricia T. ^{[3
]}

Yokosawa, Jonny ^{[4
]}

Goulart, Luiz R. ^{[1
,2
,5
]}

Neves, Adriana F. ^{[1
,2
]}

机构：

[1] Univ Fed Goias, Mol Biol Lab, Inst Phys & Chem, Catalao, Brazil

[2] Univ Fed Goias, Mol Biol Lab, Inst Biotechnol, Catalao, Brazil

[3] Univ Fed Uberlandia, Inst Genet & Biochem, Lab Nanobiotechnol, Uberlandia, MG, Brazil

[4] Univ Fed Uberlandia, Inst Biomed Sci, Lab Virol, Uberlandia, MG, Brazil

[5] Univ Calif Davis, Dept Med Microbiol & Immunol, Davis, CA 95616 USA

来源：

FUTURE MEDICINAL CHEMISTRY | 2017年 / 9卷 / 06期

关键词：

dot-blot; polymerase chain reaction; reverse transcription; SELEX; untranslated region; RNA-SYNTHESIS; ELEMENT; PROTEIN; SELECTION; CANCER;

D O I：

10.4155/fmc-2016-0233

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Aim: The dengue virus is responsible for a high worldwide incidence of infections, aggravated by late diagnosis, and often confused with other tropical diseases. Results/methodology: Oligonucleotide aptamers binding to the 5'-UTR from dengue virus selected after eight rounds by systematic evolution of ligands by exponential enrichment technology were analyzed by dot-blot assay and in silico prediction of secondary structures, demonstrating the presence of stem-loops that may have the potential for interaction with the viral genome, which can lead to loss of their original conformation. Conclusion: This is the first description of RNA aptamers against functional RNA elements of the dengue virus genome with implications for disease control, which may have potential as tools in the future of antiviral therapies and for diagnostics.

引用

页码：541 / 552

页数：12

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