Anaerobic digestion of thermal pre-treated emulsified slaughterhouse wastes (TESW): Effect of trace element limitation on process efficiency and sludge metabolic properties

Slaughterhouse solid wastes, characterized by a high lipid content, are considered a valuable resource for energy production by means of anaerobic digestion technologies. Aim of this study was to examine the effect of trace element limitation on the mesophilic anaerobic digestion of thermally pre-treated emulsified slaughterhouse wastes (TESW). Under two distinct experimental periods (Period I - low and Period II - high trace element dosage respectively) a CSTR with sludge recirculation was operated at increasing organic loading rate (OLR) from 1.5 to 10 g L-1 d(-1). Under optimum conditions, COD removal was higher than 96%, biogas yield equal to 0.53 L g(-1) COD feed and the biogas methane content 77%. Trace element limitation however, resulted in a dramatic decline in process efficiency, with VFA accumulation and events of extreme sludge flotation, despite that the soluble concentration of Ni, Co and Mo were between 12 and 28 mu g L-1. This is indicative of mass transfer limitations caused by lipids adsorption onto the anaerobic biomass. (C) 2018 Elsevier Ltd. All rights reserved.