Supercritical CO2 extraction of volatile oils from Sardinian Foeniculum vulgare ssp vulgare (Apiaceae): chemical composition and biological activity

被引：15

作者：

Piras, Alessandra ^{[1
]}

Falconieri, Danilo ^{[2
]}

Porcedda, Silvia ^{[1
]}

Marongiu, Bruno ^{[1
]}

Goncalves, Maria Jose ^{[3
,4
]}

Cavaleiro, Carlos ^{[3
,4
]}

Salgueiro, Ligia ^{[3
,4
]}

机构：

[1] Univ Cagliari, Dipartimento Sci Chim & Geol, I-09042 Monserrato, Italy

[2] Ist Tecn Ind Statale Michele Giua, I-09100 Cagliari, Italy

[3] Univ Coimbra, Fac Farm, CEF, P-3000548 Coimbra, Portugal

[4] Univ Coimbra, CNC, P-3000548 Coimbra, Portugal

来源：

NATURAL PRODUCT RESEARCH | 2014年 / 28卷 / 21期

关键词：

Foeniculum vulgare; essential oil; supercritical carbon dioxide extract; antifungal activity; CARBON-DIOXIDE EXTRACTION; ANTIFUNGAL ACTIVITY; L;

D O I：

10.1080/14786419.2014.948874

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This article reports the results on the composition and antifungal effect of volatile extracts obtained from the aerial parts of Sardinian wild fennel (Foeniculum vulgare Mill.), by supercritical fluid extraction (SFE) and by hydrodistillation (HD). The extracts were analysed by gas chromatography-mass spectrometry for qualitative composition and gas chromatography-flame ionisation detector to establish the percentage of constituents. The main components were fenchone (7.1% vs. 8.8%), estragole (34.9% vs. 42.6%) and (E)-anethole (24.6% vs. 43.4%) in the SFE and HD extract, respectively. Minimum inhibitory concentrations (MICs) were measured according to the reference Clinical and Laboratory Standards Institute (CLSI) broth macrodilution protocols. Minimum lethal concentrations were determined by subsequent subculturing of the same cell suspensions in solid medium. The essential oil was more active against Candida albicans, whereas the supercritical fluid extract possesses higher activity against Candida guillermondii and Cryptococcus neoformans, with MIC values of 0.32 mu L/mL.

引用

页码：1819 / 1825

页数：7

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