Characteristics and cellular effects of ambient particulate matter from Beijing

被引：29
作者：
Lu, Yan [1 ]
Su, Shu [1 ]
Jin, Wenjie [1 ]
Wang, Bin [1 ]
Li, Ning [2 ]
Shen, Huizhong [1 ]
Li, Wei [1 ]
Huang, Ye [1 ]
Chen, Han [1 ]
Zhang, Yanyan [1 ]
Chen, Yuanchen [1 ]
Lin, Nan [1 ]
Wang, Xilong [1 ]
Tao, Shu [1 ]
机构：
[1] Peking Univ, Coll Urban & Environm Sci, Lab Earth Surface Proc, Beijing 100871, Peoples R China
[2] Michigan State Univ, Dept Pathobiol & Diagnost Invest, E Lansing, MI 48824 USA
来源：
ENVIRONMENTAL POLLUTION | 2014年 / 191卷
基金：
中国国家自然科学基金;
关键词：
Particulate matter; In vitro test; Cytotoxicity; Inflammatory effect; DIESEL EXHAUST PARTICLES; AIR-POLLUTION; IN-VITRO; COARSE PARTICLES; OXIDATIVE STRESS; EPITHELIAL-CELLS; REDOX ACTIVITY; FINE; URBAN; SIZE;
D O I：
10.1016/j.envpol.2014.04.008
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
In vitro tests using human adenocarcinomic alveolar epithelial cell line A549 and small mouse monocyte-macrophage cell line J774A.1 were conducted to test toxicity of six PM (particulate matter) samples from Beijing. The properties of the samples differ significantly. The production of inflammatory cytokine (TNF-alpha for J774A.1) and chemokine (IL-8 for A549) and the level of intracellular reactive oxygen species (ROS) were used as endpoints. There was a positive correlation between water soluble organic carbon and DTT-based redox activity. Both cell types produced increased levels of inflammatory mediators and had higher level of intracelllar ROS, indicating the presence of PM-induced inflammatory response and oxidative stress, which were dose-dependent and significantly different among the samples. The releases of IL-8 from A549 and TNF-alpha. from J774A.1 were significantly correlated to PM size, Zeta potential, endotoxin, major metals, and polycyclic aromatic hydrocarbons. No correlation between ROS and these properties was identified. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码：63 / 69
页数：7
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