Determination of the Rate-Limiting Step in Fatty Acid Transport

The generally accepted model of free fatty acid (FA) transport through cellular membranes occurs in three steps, adsorption of the FA onto the membrane, translocation across the the cytosol. There still exists some dispute as to the identity of the rate-limiting step of FA transport. In the present study, sum-membrane ("flip-flop"), and subsequent desorption of the FA into frequency vibrational spectroscopy (SFVS) was used to directly phosphocholine (DSPC). The impact of SA on the physical measure the rate of stearic acid (SA) flip-flop in planar supported lipid bilayers (PSLBs) comprised of 1,2-distearoyl-sn-glycero-3-properties of binary mixtures of SA and DSPC was investigated via Pi-A isotherms from which the excess free energies of mixing and compression moduli were calculated. The manner in which these physical changes influenced the rates of SA and DSPC flip-flop was subsequently examined using SFVS. The rates of SA and DSPC flip-flop revealed that SA flip-flops independently of DSPC and on much faster time scales than its phospholipid counterpart. SFVS was also used to probe the rate of protein-unassisted SA desorption from hybrid supported lipid bilayers (HSLBs), allowing for the first decoupled measurement of the rates of desorption and flip-flop. These results provide strong evidence for desorption being the rate-limiting step, in FA transport through the membrane in the absence of proteins.