MicroRNA-2355-5p regulates γ-globin expression in human erythroid cells by inhibiting KLF6

被引：12

作者：

Cheng, Yi ^{[1
]}

Shang, Xuan ^{[1
,2
,3
]}

Chen, Diyu ^{[1
]}

Pang, Dejian ^{[1
]}

Zhao, Cunyou ^{[1
]}

Xu, Xiangmin ^{[1
,2
,3
]}

机构：

[1] Southern Med Univ, Sch Basic Med Sci, Dept Med Genet, Guangzhou 510515, Guangdong, Peoples R China

[2] Guangdong Genet Testing Engn Res Ctr, Guangzhou, Guangdong, Peoples R China

[3] Guangdong Prov Key Lab Single Cell Technol & Appl, Guangzhou, Guangdong, Peoples R China

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 2021年 / 193卷 / 02期

关键词：

KLF6; γ ‐ globin; miR‐ 2355‐ 5p; HbF; β thalassaemia; THALASSEMIA; INDUCTION;

D O I：

10.1111/bjh.17134

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Kruppel-like factors (KLFs) are a highly conserved family of transcription factors. We analysed expression profile data of KLFs and identified KLF6 as a new potential regulator of erythropoiesis. Knocking down the expression of KLF6 significantly raised gamma-globin mRNA and protein levels in the erythroid cell line HUDEP-2 and haematopoietic progenitor (CD34(+)) cells. We found that overexpression of microRNA (miR)-2355-5p in HUDEP-2 and CD34(+) cells correlated with increased gamma-globin synthesis by suppressing expression of KLF6. Our discovery that the interaction between miR-2355-5p and KLF6 affects the expression of gamma-globin may provide more information for the clinical management of beta-thalassaemia patients.

引用

页码：401 / 405

页数：5

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