FGF, Insulin, and SMAD Signaling Cooperate for Avian Primordial Germ Cell Self-Renewal

被引:144
作者
Whyte, Jemima [1 ,2 ]
Glover, James D. [1 ,2 ]
Woodcock, Mark [1 ,2 ]
Brzeszczynska, Joanna [1 ,2 ]
Taylor, Lorna [1 ,2 ]
Sherman, Adrian [1 ,2 ]
Kaiser, Pete [1 ,2 ]
McGrew, Michael J. [1 ,2 ]
机构
[1] Univ Edinburgh, Roslin Inst, Roslin EH25 9RG, Midlothian, Scotland
[2] Univ Edinburgh, Royal Dick Sch Vet Studies, Roslin EH25 9RG, Midlothian, Scotland
来源
STEM CELL REPORTS | 2015年 / 5卷 / 06期
基金
英国生物技术与生命科学研究理事会;
关键词
EMBRYONIC STEM-CELLS; LEUKEMIA INHIBITORY FACTOR; SERUM-FREE MEDIUM; GROWTH-FACTOR; IN-VITRO; RECEPTOR; MOUSE; SURVIVAL; NANOG; CULTURE;
D O I
10.1016/j.stemcr.2015.10.008
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Precise self-renewal of the germ cell lineage is fundamental to fertility and reproductive success. The early precursors for the germ lineage, primordial germ cells (PGCs), survive and proliferate in several embryonic locations during their migration to the embryonic gonad. By elucidating the active signaling pathways in migratory PGCs in vivo, we were able to create culture conditions that recapitulate this embryonic germ cell environment. In defined medium conditions without feeder cells, the growth factors FGF2, insulin, and Activin A, signaling through their cognate-signaling pathways, were sufficient for self-renewal of germline-competent PGCs. Forced expression of constitutively active MEK1, AKT, and SMAD3 proteins could replace their respective upstream growth factors. Unexpectedly, we found that BMP4 could replace Activin A in non-clonal growth conditions. These defined medium conditions identify the key molecular pathways required for PGC self-renewal and will facilitate efforts in biobanking of chicken genetic resources and genome editing.
引用
收藏
页码:1171 / 1182
页数:12
相关论文
共 68 条
[1]   Chicken Dead End Homologue Protein is a Nucleoprotein of Germ Cells Including Primordial Germ Cells [J].
Aramaki, Shinya ;
Kubota, Kaiyu ;
Soh, Tomoki ;
Yamauchi, Nobuhiko ;
Hattori, Masa-aki .
JOURNAL OF REPRODUCTION AND DEVELOPMENT, 2009, 55 (02) :214-218
[2]   IGF and FGF cooperatively establish the regulatory stem cell niche of pluripotent human cells in vitro [J].
Bendall, Sean C. ;
Stewart, Morag H. ;
Menendez, Pablo ;
George, Dustin ;
Vijayaragavan, Kausalia ;
Werbowetski-Ogilvie, Tamra ;
Ramos-Mejia, Veronica ;
Rouleau, Anne ;
Yang, Jiabi ;
Bosse, Marc ;
Lajoie, Gilles ;
Bhatia, Mickie .
NATURE, 2007, 448 (7157) :1015-U3
[3]   The role of cadherins during primordial germ cell migration and early gonad formation in the mouse [J].
Bendel-Stenzel, MR ;
Gomperts, M ;
Anderson, R ;
Heasman, J ;
Wylie, C .
MECHANISMS OF DEVELOPMENT, 2000, 91 (1-2) :143-152
[4]   Predictors of success of semen cryopreservation in chickens [J].
Blesbois, E. ;
Grasseau, I. ;
Seigneurin, F. ;
Mignon-Grasteau, S. ;
Saint Jalme, M. ;
Mialon-Richard, M. M. .
THERIOGENOLOGY, 2008, 69 (02) :252-261
[5]   Kit/stem cell factor receptor-induced activation of phosphatidylinositol 3′-kinase is essential for male fertility [J].
Blume-Jensen, P ;
Jiang, GQ ;
Hyman, R ;
Lee, KF ;
O'Gorman, S ;
Hunter, T .
NATURE GENETICS, 2000, 24 (02) :157-162
[6]   OPTIMIZED SURVIVAL OF HIPPOCAMPAL-NEURONS IN B27-SUPPLEMENTED NEUROBASAL(TM), A NEW SERUM-FREE MEDIUM COMBINATION [J].
BREWER, GJ ;
TORRICELLI, JR ;
EVEGE, EK ;
PRICE, PJ .
JOURNAL OF NEUROSCIENCE RESEARCH, 1993, 35 (05) :567-576
[7]   SB-505124 is a selective inhibitor of transforming growth factor-β type I receptors ALK4, ALK5, and ALK7 [J].
Byfield, SD ;
Major, C ;
Laping, NJ ;
Roberts, AB .
MOLECULAR PHARMACOLOGY, 2004, 65 (03) :744-752
[8]   Germ cell restricted expression of chick nanog [J].
Canon, Susana ;
Herranz, Cristina ;
Manzanares, Miguel .
DEVELOPMENTAL DYNAMICS, 2006, 235 (10) :2889-2894
[9]   Nanog safeguards pluripotency and mediates germline development [J].
Chambers, Ian ;
Silva, Jose ;
Colby, Douglas ;
Nichols, Jennifer ;
Nijmeijer, Bianca ;
Robertson, Morag ;
Vrana, Jan ;
Jones, Ken ;
Grotewold, Lars ;
Smith, Austin .
NATURE, 2007, 450 (7173) :1230-U8
[10]   Stochastic specification of primordial germ cells from mesoderm precursors in axolotl embryosStochastic specification of primordial germ cells from mesoderm precursors in axolotl embryos [J].
Chatfield, Jodie ;
O'Reilly, Marie-Anne ;
Bachvarova, Rosemary F. ;
Ferjentsik, Zoltan ;
Redwood, Catherine ;
Walmsley, Maggie ;
Patient, Roger ;
Loose, Mathew ;
Johnson, Andrew D. .
DEVELOPMENT, 2014, 141 (12) :2429-2440