The effect of S100A6 on nuclear translocation of CacyBP/SIP in colon cancer cells

被引：7

作者：

Feng, Shanshan ^{[1
]}

Zhou, Qiaozhi ^{[2
]}

Yang, Bo ^{[2
]}

Li, Qianqian ^{[2
]}

Liu, Aiqin ^{[2
]}

Zhao, Yingying ^{[2
]}

Qiu, Changqing ^{[2
]}

Ge, Jun ^{[2
]}

Zhai, Huihong ^{[2
,3
,4
]}

机构：

[1] Ningxia Med Univ, Gen Hosp, Surg Lab, Yinchuan, Ningxia Hui Aut, Peoples R China

[2] Capital Med Univ, Beijing Friendship Hosp, Dept Gastroenterol, Beijing, Peoples R China

[3] Beijing Key Lab Precancerous Les Digest Dis, Beijing, Peoples R China

[4] Natl Clin Res Ctr Digest Dis, Beijing, Peoples R China

来源：

PLOS ONE | 2018年 / 13卷 / 03期

基金：

中国国家自然科学基金;

关键词：

CALCYCLIN-BINDING-PROTEIN; GASTRIC-CANCER; INTERACTING PROTEIN; EXPRESSION; SIAH-1; TARGET;

D O I：

10.1371/journal.pone.0192208

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Background Calcyclin Binding Protein/(Siah-1interacting protein) (CacyBP/SIP) acts as an oncogene in colorectal cancer. The nuclear accumulation of CacyBP/SIP has been linked to the proliferation of cancer cells. It has been reported that intracellular Ca2+ induces the nuclear translocation of CacyBP/SIP. However, the molecular mechanism of CacyBP/SIP nuclear translocation has yet to be elucidated. The purpose of this study was to test whether the Cat(+)-dependent binding partner S100 protein is involved in CacyBP/SIP nuclear translocation in colon cancer SW480 cells. Methods The subcellular localization of endogenous CacyBP/SIP was observed following the stimulation of ionomycin or BAPTA/AM by immunofluorescence staining in SW480 cells. S100A6 small interfering RNAs (siRNA) were transfected into SW480 cells. Immunoprecipitation assays detected whether S100 protein is relevant to the nuclear translocation of CacyBP/SIP in response to changes in [Ca2+]/. Results We observed that endogenous CacyBP/SIP is translocated from the cytosol to the nucleus following the elevation of [Ca2+]/by ionomycin in SW480 cells. Co-immunoprecipitation experiments showed that the interaction between S100A6 and CacyBP/SIP was increased simultaneously with elevated Ca2+. Knockdown of S100A6 abolished the Ca2+ effect on the subcellular translocation of CacyBP/SIP. Conclusion Thus, we demonstrated that S100A6 is required for the Ca2+-dependent nuclear translocation of CacyBP/SIP in colon cancer SW480 cells.

引用

页数：13

共 17 条

[1] Molecular cloning and expression of a mouse brain cDNA encoding a novel protein target of calcyclin [J].

Filipek, A ;

Kuznicki, J .

JOURNAL OF NEUROCHEMISTRY, 1998, 70 (05) :1793-1798

[2] Ca2+-dependent translocation of the calcyclin-binding protein in neurons and neuroblastoma NB-2a cells [J].

Filipek, A ;

Jastrzebska, B ;

Nowotny, M ;

Kwiatkowska, K ;

Hetman, M ;

Surmacz, L ;

Wyroba, E ;

Kuznicki, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (23) :21103-21109

[3] CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family [J].

Filipek, A ;

Jastrzebska, B ;

Nowotny, M ;

Kuznicki, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (32) :28848-28852

[4] Effects of Repetitive Ionomycin Treatment on In Vitro Development of Bovine Somatic Cell Nuclear Transfer Embryos [J].

Kim, Huen Suk ;

Lee, Jong Yun ;

Jeong, Eun Ji ;

Yang, Chi Jeon ;

Hyun, Sang Hwan ;

Shin, Taeyoung ;

Hwang, Woo Suk .

JOURNAL OF REPRODUCTION AND DEVELOPMENT, 2012, 58 (01) :132-139

[5]

Leclerc Estelle, 2011, Front Biosci (Schol Ed), V3, P1232

[6] Structure of the S100A6 complex with a fragment from the C-terminal domain of Siah-1 interacting protein: A novel mode for S100 protein target recognition [J].

Lee, Young-Tae ;

Dimitrova, Yoana N. ;

Schneider, Gabriela ;

Ridenour, Whitney B. ;

Bhattacharya, Shibani ;

Soss, Sarah E. ;

Caprioli, Richard M. ;

Filipek, Anna ;

Chazin, Walter J. .

BIOCHEMISTRY, 2008, 47 (41) :10921-10932

[7] S100A6-New facts and features [J].

Lesniak, Wieslawa ;

Slomnicki, Lukasz P. ;

Filipek, Anna .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 390 (04) :1087-1092

[8] Siah-1, SIP, and Ebi collaborate in a novel pathway for β-catenin degradation linked to p53 responses [J].

Matsuzawa, S ;

Reed, JC .

MOLECULAR CELL, 2001, 7 (05) :915-926

[9]

Ning X. X., 2005, THESIS

[10] CacyBP/SIP nuclear translocation regulates p27Kip1 stability in gastric cancer cells [J].

Niu, Ying-Lin ;

Li, Ya-Jun ;

Wang, Jing-Bo ;

Lu, Yuan-Yuan ;

Liu, Zhen-Xiong ;

Feng, Shan-Shan ;

Hu, Jian-Guo ;

Zhai, Hui-Hong .

WORLD JOURNAL OF GASTROENTEROLOGY, 2016, 22 (15) :3992-4001

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