Colonization of rice straw by white-rot fungi (Cyathus stercoreus): Effect on ruminal fermentation pattern, nitrogen metabolism, and fiber utilization during continuous culture

被引:41
作者
Karunanandaa, K [1 ]
Varga, GA [1 ]
机构
[1] PENN STATE UNIV,DEPT DAIRY & ANIM SCI,UNIVERSITY PK,PA 16802
关键词
cattle; rice straw; fungi;
D O I
10.1016/0377-8401(96)00958-3
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Experimental diets consisted of fungal treated or untreated lice straw: concentrate 75:25 on a DM basis and ground through a 1 mm sieve. Fungal treated rice straw (FRS) and untreated rice straw (C) diets were formulated to be isonitrogenous (11% crude protein; CP), but varied in total nonstructural carbohydrates, neutral detergent fiber and acid detergent fiber (18.3 vs, 11.4%; 49.7 vs. 61.0%; 45.0 vs. 47.5%), respectively. Four fermenters were inoculated with ruminal fluid from a ruminally cannulated lactating Holstein cow fed a total mixed ration consisting of 50% silage: 50% grain (DM basis). A randomized complete block design was used with diets replicated within each experimental period and four times across periods, Each period was 10 days in length, 7 days for adaptation followed by 3 days for sample collection. Digesta solid and liquid dilution rates were 0.055 h(-1). Apparent and true digestion of DM and OM of the FRS-diet was higher (P < 0.05) compared with the C-diet. Fungal treatment increased (P < 0.05) the digestibility of the major fiber component, cellulose, compared to the C-diet (61.1 vs, 48.8%). Actual quantities of nutrients digested per unit rime indicated that fungal treatment increased cellulose digestion by 27% but decreased hemicellulose digestion by 37%, when compared with the C-diet. Arabinose and xylose from FRS-diet were less digestible, however glucose digestion was increased by 38% compared with the C-diet, Increased digestion of carbohydrates from FRS-diet resulted in an increase (P < 0.05) on production of total volatile fatty acids (VFA) compared with the C-diet. Increased VFA production of FRS-diet was due to increased molar proportion (moles 100 moles(-1)) of propionate and butyrate (28.8 vs. 24.2; 13.1 vs. 7.9), respectively. Branched chain VFA production was not detectable with the FRS-diet when compared with an average of 2.6 moles 100 moles(-1) for the C-diet. A similar pattern of inhibition was found with ammonia-N production with FRS-diet compared with the C-diet (0.77 vs. 22.3 mg dl(-1)). Nitrogen metabolism of FRS-diet resulted in lower true digestion of CP and microbial protein synthesis compared with the C-diet, resulting in increased dietary-N flow. This study demonstrated that fungal treatment increased fiber digestibility by increasing the availability of cellulose but decreasing CP availability for rumen microbial digestion.
引用
收藏
页码:1 / 16
页数:16
相关论文
共 44 条
[1]   FUNGAL PRETREATMENT OF WHEAT STRAW - EFFECTS ON THE BIODEGRADABILITY OF CELL-WALLS, STRUCTURAL POLYSACCHARIDES, LIGNIN AND PHENOLIC-ACIDS BY RUMEN MICROORGANISMS [J].
AGOSIN, E ;
TOLLIER, MT ;
BRILLOUET, JM ;
THIVEND, P ;
ODIER, E .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1986, 37 (02) :97-106
[2]   MICROBIAL DELIGNIFICATION WITH WHITE-ROT FUNGI IMPROVES FORAGE DIGESTIBILITY [J].
AKIN, DE ;
SETHURAMAN, A ;
MORRISON, WH ;
MARTIN, SA ;
ERIKSSON, KEL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (12) :4274-4282
[3]  
BELLOMONTE G, 1987, J ASSOC OFF ANA CHEM, V70, P227
[4]   A SIMPLE AND RAPID PREPARATION OF ALDITOL ACETATES FOR MONOSACCHARIDE ANALYSIS [J].
BLAKENEY, AB ;
HARRIS, PJ ;
HENRY, RJ ;
STONE, BA .
CARBOHYDRATE RESEARCH, 1983, 113 (02) :291-299
[5]   ACCEPTABILITY, BODY-WEIGHT CHANGES AND DIGESTIBILITY OF SPENT WHEAT STRAW AFTER HARVESTING OF PLEUROTUS-SAJOR-CAJU [J].
CALZADA, JF ;
FRANCO, LF ;
DEARRIOLA, MC ;
ROLZ, C ;
ORTIZ, MA .
BIOLOGICAL WASTES, 1987, 22 (04) :303-309
[6]  
CHANDRA S, 1991, INDIAN J ANIM SCI, V61, P1330
[7]  
Eggeling L, 1983, TRENDS BIOTECHNOL, V1, P123
[8]  
EICKELBERGER RC, 1983, THESIS PENNSYLVANIA
[9]  
FAHEY GC, 1993, FORAGE CELL WALL STRUCTURE AND DIGESTIBILITY, P715
[10]  
GIOVANNOZZISERMANNI G, 1990, ADVANCES IN BIOLOGICAL TREATMENT OF LIGNOCELLULOSIC MATERIALS, P59