DNA Binding and Oxidative Cleavage Studies of Mixed-ligand Copper(II) Complex

被引：1
作者：
Tang, Siping [1 ,2 ]
Feng, Yonglan [1 ,2 ]
Xu, Zhifeng [1 ,2 ]
Yang, Yingqun [1 ,2 ]
Wang, Deping [1 ,2 ]
机构：
[1] Hengyang Normal Univ, Dept Chem & Mat Sci, Hengyang 421008, Hunan, Peoples R China
[2] Hengyang Normal Univ, Coll Hunan Prov, Key Lab Funct Organometall Mat, Hengyang 421008, Hunan, Peoples R China
来源：
ASIAN JOURNAL OF CHEMISTRY | 2013年 / 25卷 / 16期
基金：
中国国家自然科学基金;
关键词：
Mixed-ligand copper(II) complex; DNA binding; Nuclease activity; BRIDGED DICOPPER(II) COMPLEXES; HETEROCYCLIC BASES; CRYSTAL-STRUCTURE; NUCLEASE ACTIVITY; STRAND SCISSION; ARTIFICIAL METALLONUCLEASES; CHEMICAL NUCLEASES; 1,10-PHENANTHROLINE; OXYGEN; ACIDS;
D O I：
10.14233/ajchem.2013.15191
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A mixed-ligand copper(II) complex ((4'-ferrocenyl-2,2': 6', 2''-terpyridine-k(3),N',N'') (1,1'-phenanthroline-k(2)N',N")-copper(II) bis(perchlorate)) has been synthesized. Binding interactions of the complex with calf thymus DNA (CT-DNA) were investigated by UV-visible and CD spectroscopies. Results show that the copper complex mainly adopt electrostatic attraction binding mode with DNA and the binding constant is (3.22 +/- 0.05) x 10(3) mol(-1) L. The complex shows appreciable chemical nuclease activity in the presence of ascorbic acid. The DNA cleavage by the complex were inhibited strongly in the presence of DMSO and tert-butyl alcohol, which suggests that hydroxyl radicals are reactive oxygen species for the cleavage.
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页码：9237 / 9240
页数：4
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