Solvent-Free Fabrication of Tissue Engineering Scaffolds With Immiscible Polymer Blends

被引：20

作者：

Ma, Liang ^{[1
,2
]}

Jiang, Wei ^{[1
]}

Li, Wei ^{[1
]}

机构：

[1] Univ Texas Austin, Dept Mech Engn, Austin, TX 78712 USA

[2] Zhejiang Univ, Zhejiang Calif Int NanoSyst Inst, Hangzhou, Zhejiang, Peoples R China

来源：

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS | 2014年 / 63卷 / 10期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

pore size; tissue engineering scaffolds; porosity; immiscible polymer; solvent-free fabrication; GBM cells; INDUCED PHASE-SEPARATION; BIODEGRADABLE POLYMERS; LEACHING TECHNIQUES; FOAMS; CELLS; TRANSPLANTATION; COMBINATION; MORPHOLOGY; REPAIR; MODEL;

D O I：

10.1080/00914037.2013.854222

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

A completely organic solvent-free fabrication method is developed for tissue engineering scaffolds by gas foaming of immiscible polylactic acid (PLA) and sucrose blends, followed by water leaching. PLA scaffolds with above 90% porosity and 25-200 mu m pore size were fabricated. The pore size and porosity was controlled with process parameters including extrusion temperature and foaming process parameters. Dynamic mechanical analysis showed that the extrusion temperature could be used to control the scaffold strength. Both unfoamed and foamed scaffolds were used to culture glioblastoma (GBM) cells M059K. The results showed that the cells grew better in the foamed PLA scaffolds. The method presented in the paper is versatile and can be used to fabricate tissue engineering scaffolds without any residual organic solvents.

引用

页码：510 / 517

页数：8

共 39 条

[1] Solvent-free fabrication of three dimensionally aligned polycaprolactone microfibers for engineering of anisotropic tissues [J].

An, Jia ;

Chua, Chee Kai ;

Leong, Kah Fai ;

Chen, Chih-Hao ;

Chen, Jyh-Ping .

BIOMEDICAL MICRODEVICES, 2012, 14 (05) :863-872

[2]

[Anonymous], 2008, D792 ASTM

[3] A novel porous cells scaffold made of polylactide-dextran blend by combining phase-separation and particle-leaching techniques [J].

Cai, Q ;

Yang, JA ;

Bei, JZ ;

Wang, SG .

BIOMATERIALS, 2002, 23 (23) :4483-4492

[4] Fabrication of PLGA Based Tissue Engineering Scaffolds via Photocuring and Salt Leaching Techniques [J].

Cicek, Cigdem ;

Cakmakci, Emrah ;

Kayaman-Apohan, Nilhan ;

Arslan, Mehmet ;

Kuruca, Serap Erdem .

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, 2013, 62 (14) :719-725

[5] Freeform fabrication of Nylon-6 tissue engineering scaffolds [J].

Das, S ;

Hollister, SJ ;

Flanagan, C ;

Adewunmi, A ;

Bark, K ;

Chen, C ;

Ramaswamy, K ;

Rose, D ;

Widjaja, E .

RAPID PROTOTYPING JOURNAL, 2003, 9 (01) :43-49

[6] Cells adapted to high NaCl have many DNA breaks and impaired DNA repair both in cell culture and in vivo [J].

Dmitrieva, NI ;

Cai, Q ;

Burg, MB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (08) :2317-2322

[7]

Harris LD, 1998, J BIOMED MATER RES, V42, P396, DOI 10.1002/(SICI)1097-4636(19981205)42:3<396::AID-JBM7>3.0.CO

[8]

2-E

[9] Porous polymeric structures for tissue engineering prepared by a coagulation, compression moulding and salt leaching technique [J].

Hou, QP ;

Grijpma, DW ;

Feijen, J .

BIOMATERIALS, 2003, 24 (11) :1937-1947

[10] Polyhydroxybutyrate (PHB) Scaffolds as a Model for Nerve Tissue Engineering Application: Fabrication and In Vitro Assay [J].

Khorasani, M. T. ;

Mirmohammadi, S. A. ;

Irani, S. .

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS, 2011, 60 (08) :562-575

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