Design of Synthetic Polymer Nanoparticles that Capture and Neutralize a Toxic Peptide

被引：90

作者：

Hoshino, Yu ^{[1
]}

Urakami, Takeo ^{[2
]}

Kodama, Takashi ^{[3
]}

Koide, Hiroyuki ^{[2
]}

Oku, Naoto ^{[2
]}

Okahata, Yoshio ^{[3
]}

Shea, Kenneth J. ^{[1
]}

机构：

[1] Univ Calif Irvine, Dept Chem, Irvine, CA 92697 USA

[2] Univ Shizuoka, Dept Med Biochem, Sch Pharmaceut Sci, Shizuoka 4228526, Japan

[3] Tokyo Inst Technol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

SMALL | 2009年 / 5卷 / 13期

基金：

美国国家卫生研究院;

关键词：

antibodies; biomolecules; nanoparticles; polymers; protein recognition; QUARTZ-CRYSTAL MICROBALANCE; PROTEIN FIBRILLATION; IDENTIFICATION; TRANSITION; MICROGELS; MELITTIN;

D O I：

10.1002/smll.200900186

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Designed polymer nanoparticles (NPs) capable of binding and neutralizing a biomacromolecular toxin are prepared. A library of copolymer NPs is synthesized from combinations of functional monomers. The binding capacity and affinity of the NPs are individually analyzed. NPs with optimized composition are capable of neutralizing the toxin even in a complex biological milieu. It is anticipated that this strategy will be a starting point for the design of synthetic alternatives to antibodies.

引用

页码：1562 / 1568

页数：7

共 20 条

[1] [Anonymous], 1993, AMPHIPATHIC HELIX
[2] Blondelle SE, 1996, J BIOL CHEM, V271, P4093, DOI 10.1074/jbc.271.8.4093
[3] REVERSAL OF TOXICITY USING AVIDIN-BASED HEMOPERFUSION - A MODEL SYSTEM IN RATS USING BIOTINYLATED MELITTIN
BRITT, AM
BURKHART, KK
BILLINGSLEY, ML
[J]. PHARMACOLOGY, 1995, 50 (05) : 307 - 312
[4] Inhibition of Amyloid β Protein Fibrillation by Polymeric Nanoparticles
Cabaleiro-Lago, Celia
Quinlan-Pluck, Fiona
Lynch, Iseult
Lindman, Stina
Minogue, Aedin M.
Thulin, Eva
Walsh, Dominic M.
Dawson, Kenneth A.
Linse, Sara
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (46) : 15437 - 15443
[5] Cedervall T., 2007, Angewandte Chemie International Edition, V119, P5856, DOI DOI 10.1002/ANGE.200700465
[6] Detailed identification of plasma proteins adsorbed on copolymer nanoparticles
Cedervall, Tommy
Lynch, Iseult
Foy, Martina
Berggard, Tord
Donnelly, Seamas C.
Cagney, Gerard
Linse, Sara
Dawson, Kenneth A.
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (30) : 5754 - 5756
[7] Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles
Cedervall, Tommy
Lynch, Iseult
Lindman, Stina
Berggard, Tord
Thulin, Eva
Nilsson, Hanna
Dawson, Kenneth A.
Linse, Sara
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (07) : 2050 - 2055
[8] Synthesis and characterization of pH-responsive copolymer microgels with tunable volume phase transition temperatures
Debord, JD
Lyon, LA
[J]. LANGMUIR, 2003, 19 (18) : 7662 - 7664
[9] The dawning era of polymer therapeutics
Duncan, R
[J]. NATURE REVIEWS DRUG DISCOVERY, 2003, 2 (05) : 347 - 360
[10] A KINETIC-STUDY OF CONCANAVALIN-A BINDING TO GLYCOLIPID MONOLAYERS BY USING A QUARTZ-CRYSTAL MICROBALANCE
EBARA, Y
OKAHATA, Y
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (25) : 11209 - 11212

← 1 2 →