Isolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the edible dormouse, Glis glis

被引：10

作者：

Huerner, H. ^{[1
]}

Martin, J. F. ^{[2
]}

Ribas, A. ^{[3
,4
]}

Arrizabalaga, A. ^{[4
]}

Michaux, J. R. ^{[1
,2
]}

机构：

[1] Univ Liege, Lab Genet Microorganismes, B-4000 Liege, Belgium

[2] CBGP, UMR 1062, F-34988 Montferrier Sur Lez, France

[3] CBGP, UMR, INRA IRD Cirad Montpellier SupAgro, F-34988 Montferrier Sur Lez, France

[4] Museu Granollers Ciencies Nat C Francesc Macia, Granollers 5108402, Spain

来源：

MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷 / 03期

关键词：

dormouse; Glis; microsatellite; multiplex; rodentia;

D O I：

10.1111/j.1755-0998.2008.02365.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated and characterized 10 dinucleotide microsatellite loci in the edible dormouse, Glis glis (Linnaeus). Four multiplex panels were developed. Loci were amplified in samples from two geographically distant populations (Torgny in Belgium and Montseny in Spain). All loci were polymorphic in Spain but four were monomorphic in Belgium. Individuals from Belgium and Spain exhibited an average allelic diversity of 1.9 and 3.3 and an observed heterozygosity ranging from 0.08 to 0.47 and from 0.04 to 0.72, respectively.

引用

页码：885 / 887

页数：3

共 8 条

[1] Microsatellite null alleles and estimation of population differentiation
Chapuis, Marie-Pierre
Estoup, Arnaud
[J]. MOLECULAR BIOLOGY AND EVOLUTION, 2007, 24 (03) : 621 - 631
[2] MAXIMUM LIKELIHOOD FROM INCOMPLETE DATA VIA EM ALGORITHM
DEMPSTER, AP
LAIRD, NM
RUBIN, DB
[J]. JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-METHODOLOGICAL, 1977, 39 (01): : 1 - 38
[3] Polymorphic microsatellite loci and PCR multiplexing in the common vole, Microtus arvalis
Gauffre, B.
Galan, M.
Bretagnolle, V.
Cosson, J. F.
[J]. MOLECULAR ECOLOGY NOTES, 2007, 7 (05): : 830 - 832
[4] Kijas J. M. H., 1994, Biotechniques, V16, P657
[5] Krystufek B., 1999, ATLAS EUROPEAN MAMMA, P294
[6] GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM
RAYMOND, M
ROUSSET, F
[J]. JOURNAL OF HEREDITY, 1995, 86 (03) : 248 - 249
[7] Schneider SR., 2000, GENETICS BIOMETRY LA
[8] STORCH C, 1978, HDB SAUGETIERE EURO, P243

← 1 →