Super-Resolution Biological Microscopy Using Virtual Imaging by a Microsphere Nanoscope

被引：179

作者：

Yang, Hui ^{[1
]}

Moullan, Norman ^{[2
]}

Auwerx, Johan ^{[2
]}

Gijs, Martin A. M. ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Microsyst, CH-1015 Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Lab Integrat & Syst Physiol, CH-1015 Lausanne, Switzerland

来源：

SMALL | 2014年 / 10卷 / 09期

基金：

瑞士国家科学基金会;

关键词：

fluorescence; imaging; microsphere nanoscope; super resolution; PHOTONIC-NANOJETS; DIFFRACTION-LIMIT; RESOLUTION; LENSES; ENHANCEMENT; CENTROSOME; SUPERLENS; DISEASE; LIGHT;

D O I：

10.1002/smll.201302942

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Super-resolution imaging of sub-cellular organelles and nanostructures is achieved by simply putting high-refractive index glass microspheres on a sample in combination with a microscope immersion objective for observation. The microsphere projects the sample's near-field nanoscopic features into the far-field, generating a magnified virtual image and demonstrating a resolution four times better than that corresponding to the classical diffraction limit. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：1712 / 1718

页数：7

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