Super-Resolution Biological Microscopy Using Virtual Imaging by a Microsphere Nanoscope

被引：173
|
作者：
Yang, Hui [1 ]
Moullan, Norman [2 ]
Auwerx, Johan [2 ]
Gijs, Martin A. M. [1 ]
机构：
[1] Ecole Polytech Fed Lausanne, Lab Microsyst, CH-1015 Lausanne, Switzerland
[2] Ecole Polytech Fed Lausanne, Lab Integrat & Syst Physiol, CH-1015 Lausanne, Switzerland
来源：
SMALL | 2014年 / 10卷 / 09期
基金：
瑞士国家科学基金会;
关键词：
fluorescence; imaging; microsphere nanoscope; super resolution; PHOTONIC-NANOJETS; DIFFRACTION-LIMIT; RESOLUTION; LENSES; ENHANCEMENT; CENTROSOME; SUPERLENS; DISEASE; LIGHT;
D O I：
10.1002/smll.201302942
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Super-resolution imaging of sub-cellular organelles and nanostructures is achieved by simply putting high-refractive index glass microspheres on a sample in combination with a microscope immersion objective for observation. The microsphere projects the sample's near-field nanoscopic features into the far-field, generating a magnified virtual image and demonstrating a resolution four times better than that corresponding to the classical diffraction limit. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：1712 / 1718
页数：7
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