Characteristics of Cardiac Cell Cultures Derived from Human Myocardial Explants

被引：3

作者：

Pavlova, S. V. ^{[1
,2
,3
]}

Perovskii, P. P. ^{[1
,2
,3
]}

Chepeleva, E. V. ^{[1
,2
,3
]}

Malakhova, A. A. ^{[1
,2
,3
]}

Dement'eva, E. V. ^{[1
,2
,3
]}

Pokushalov, E. A. ^{[2
,3
]}

Sukhikh, G. T. ^{[4
]}

Zakiyan, S. M. ^{[1
,2
,3
]}

机构：

[1] Russian Acad Sci, Inst Cytol & Genet, Siberian Div, Moscow, Russia

[2] Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Div, Novosibirsk, Russia

[3] Minist Hlth Russian Federat, EN Meshalkin Res Inst Circulatory Pathol, Novosibirsk, Russia

[4] Minist Hlth Russian Federat, VI Kulakov Res Ctr Obstet Gynecol & Perinatol, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2013年 / 156卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

cardiac stem cells; endotheliocytes; pericytes; myofibroblasts; angiogenesis; STEM-CELLS; ISCHEMIC CARDIOMYOPATHY; IN-VITRO; PERICYTES; CARDIOSPHERES; FIBROBLASTS; MULTIPOTENT; EXPRESSION; HEART;

D O I：

10.1007/s10517-013-2295-x

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Primary cell cultures derived from human myocardial explants were obtained and characterized. The explant cultures contained cardiac stem cells (c-kit(+); similar to 4%), microvascular cells (endothelial cells and pericytes), fibroblasts, and myofibroblasts. It was demonstrated that culturing of cardiac cells in cardiospheres did not promote enrichment of the cell culture with stem cells. MACS-sorted c-kit(+) cells from the explant culture were characterized by limited proliferative capacity and were capable of cardiomyogenic differentiation. The presence of microvascular cells determined general angiogenic potential of the culture.

引用

页码：127 / 135

页数：9

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