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Apoptosis is an obstacle to the differentiation of adipose-derived stromal cells into astrocytes
被引：3
|作者：
Yuan, Xiaodong
[1
]
Sun, Qiaoyu
[1
]
Ou, Ya
[1
]
Wang, Shujuan
[1
]
Zhang, Wenli
[2
]
Deng, Hongliang
[1
]
Wu, Xiaoying
[1
]
Zhang, Lili
[1
]
机构：
[1] Hebei United Univ, Kailuan Gen Hosp, Dept Neurol, Tangshan 063000, Hebei Province, Peoples R China
[2] Hebei United Univ, Dept Electron Microscopy, Tangshan 063000, Hebei Province, Peoples R China
关键词：
nerve regeneration;
adult adipose-derived stromal cells;
cell apoptosis;
caspase-dependent apoptosis;
cell differentiation;
astrocytes;
caspase-9;
caspase-3;
neural regeneration;
CASPASE-DEPENDENT APOPTOSIS;
MESENCHYMAL STEM-CELLS;
MOTOR-NEURONS;
BONE-MARROW;
IN-VITRO;
INDEPENDENT APOPTOSIS;
THYMOCYTE APOPTOSIS;
ALZHEIMERS-DISEASE;
REACTIVE GLIOSIS;
CANCER-CELLS;
D O I：
10.4103/1673-5374.131600
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ;
090102 ;
摘要：
Previous studies have demonstrated that nerve cells differentiated from adipose-derived stromal cells after chemical induction have reduced viability; however, the underlying mechanisms remained unclear. In this study, we induced the differentiation of adult adipose-derived stromal cells into astrocytes using chemical induction. 3-(4,5-Dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide assay and flow cytometry showed that, with increasing induction time, the apoptotic rate gradually increased, and the number of living cells gradually decreased. Immunohistochemical staining demonstrated that the number of glial fibrillary acidic protein-, caspase-3- and caspase-9-positive cells gradually increased with increasing induction time. Transmission electron microscopy revealed typical signs of apoptosis after differentiation. Taken together, our results indicate that caspase-dependent apoptosis is an obstacle to the differentiation of adipose-derived stromal cells into astrocytes. Inhibiting apoptosis may be an important strategy for increasing the efficiency of induction.
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页码：837 / 844
页数：8
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