A Petunia Homeodomain-Leucine Zipper Protein, PhHD-Zip, Plays an Important Role in Flower Senescence

被引：50
作者：
Chang, Xiaoxiao [1 ,2 ]
Donnelly, Linda [3 ]
Sun, Daoyang [1 ,2 ]
Rao, Jingping [1 ]
Reid, Michael S. [2 ]
Jiang, Cai-Zhong [3 ]
机构：
[1] Northwest A&F Univ, Dept Hort, Yangling, Shaanxi, Peoples R China
[2] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
[3] USDA, Crops Pathol & Genet Res Unit, ARS, Davis, CA USA
来源：
PLOS ONE | 2014年 / 9卷 / 02期
基金：
美国农业部;
关键词：
ABSCISIC-ACID; ARABIDOPSIS-THALIANA; WATER-DEFICIT; ETHYLENE BIOSYNTHESIS; SENESCING PETALS; ENDOGENOUS ABA; SHOOT GROWTH; DNA-BINDING; GENES; TRANSCRIPTION;
D O I：
10.1371/journal.pone.0088320
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Flower senescence is initiated by developmental and environmental signals, and regulated by gene transcription. A homeodomain-leucine zipper transcription factor, PhHD-Zip, is up-regulated during petunia flower senescence. Virus-induced gene silencing of PhHD-Zip extended flower life by 20% both in unpollinated and pollinated flowers. Silencing PhHD-Zip also dramatically reduced ethylene production and the abundance of transcripts of genes involved in ethylene (ACS, ACO), and ABA (NCED) biosynthesis. Abundance of transcripts of senescence-related genes (SAG12, SAG29) was also dramatically reduced in the silenced flowers. Over-expression of PhHD-Zip accelerated petunia flower senescence. Furthermore, PhHD-Zip transcript abundance in petunia flowers was increased by application of hormones (ethylene, ABA) and abiotic stresses (dehydration, NaCl and cold). Our results suggest that PhHD-Zip plays an important role in regulating petunia flower senescence.
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页数：10
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