Characterization of teratogenic potential and gene expression in canine and feline amniotic membrane-derived stem cells

被引：24
作者：
Cardoso, M. T. [1 ]
Pinheiro, A. O. [1 ]
Vidane, A. S. [2 ]
Casals, J. B. [2 ]
de Oliveira, V. C. [2 ]
Goncalves, N. J. N. [2 ]
Martins, D. S. [1 ,2 ]
Ambrosio, C. E. [1 ,2 ]
机构：
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, SP, Brazil
[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sao Paulo, SP, Brazil
来源：
REPRODUCTION IN DOMESTIC ANIMALS | 2017年 / 52卷
基金：
巴西圣保罗研究基金会;
关键词：
foetal membranes; oncology; safety; VITRO DIFFERENTIATION POTENCY;
D O I：
10.1111/rda.12832
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Contents The biosafety of innovative procedures that utilize stem cells in regenerative medicine has been addressed in several studies. Previous work has showed no tumour formation following the use of feline and human amniotic membrane-derived stem cells (AMSCs). In contrast, tumour formation was observed when canine AMSCs were utilized. These findings suggested that feline and human, but not canine, AMSCs are suitable for cell transplantation trials. This study aimed to further evaluate the feasibility of utilizing canine AMSCs for transplantation purposes as well as for felines. We tested teratoma formation following cell injection into BALB/c nude mice and then assessed expression of haematopoietic, mesenchymal, tumorigenic, pluripotency and cellular regulation markers using flow cytometry and qPCR. The use of canine AMSCs did not result in macroscopic tumour formation as determined 60days after transplantation. The immunophenotypic characterization by flow cytometry revealed expression of mesenchymal markers (CD73 and CD90) and expression of the pluripotent marker OCT4 and SOX2. Quantitative PCR analysis revealed that there were no differences in the patterns of gene expression (CD34, CD73, OCT4, CD30 and P53) between canine and feline AMSCs, with the exception of the expression of SOX2 and CD90.
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收藏
页码：58 / 64
页数：7
相关论文
共 20 条
  • [1] Isolation and basic characterization of human term amnion and chorion mesenchymal stromal cells
    Bacenkova, Darina
    Rosocha, Jan
    Tothova, Timea
    Rosocha, Ladislav
    Sarissky, Marek
    [J]. CYTOTHERAPY, 2011, 13 (09) : 1047 - 1056
  • [2] Evolution of cancer stem cells
    Bapat, S. A.
    [J]. SEMINARS IN CANCER BIOLOGY, 2007, 17 (03) : 204 - 213
  • [3] Beyer Nardi N, 2006, HANDB EXP PHARM, V174, P249
  • [4] Bydlowski Sergio P., 2009, Rev. Bras. Hematol. Hemoter., V31, P25
  • [5] desVita B., 2012, VET ZOOTEC-BRAZIL, V19, P8
  • [6] EVANS H E, 1973, Zentralblatt fuer Veterinaermedizin Reihe C, V2, P11
  • [7] Stem cell plasticity
    Lakshmipathy, U
    Verfaillie, C
    [J]. BLOOD REVIEWS, 2005, 19 (01) : 29 - 38
  • [8] Lima E. B., 2009, 17 ANN M INT SOC STE
  • [9] Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method
    Livak, KJ
    Schmittgen, TD
    [J]. METHODS, 2001, 25 (04) : 402 - 408
  • [10] The carnivore pregnancy:: The development of the embryo and fetal membranes
    Miglino, Maria Angelica
    Ambrosio, Carlos Eduardo
    Martins, Daniele dos Santos
    Wenceslau, Cristiane Valverde
    Pfarrer, Christiane
    Leiser, Rudolf
    [J]. THERIOGENOLOGY, 2006, 66 (6-7) : 1699 - 1702
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