Purification and Characterization of Extracellular Protease and Amylase Produced by the Bacterial Strain, Corynebacterium alkanolyticum ATH3 Isolated from Fish Gut

被引:16
作者
Banerjee, Goutam [1 ]
Mukherjee, Sandip [2 ]
Bhattacharya, Shelley [2 ]
Ray, Arun K. [1 ]
机构
[1] Visva Bharati Univ, Dept Zool, Fisheries Lab, Santini Ketan 731235, W Bengal, India
[2] Visva Bharati Univ, Dept Zool, Environm Toxicol Lab, Santini Ketan 731235, W Bengal, India
关键词
Fish gut bacteria; Protease and amylase purification; SDS-PAGE; Zymography analysis; pH and temperature sensitivity; ALPHA-AMYLASE; MODERATE HALOPHILE;
D O I
10.1007/s13369-015-1809-4
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The use of enzymes in different industrial sectors increased significantly due to huge industrialization. Different types of protease and amylase are randomly used in industries including food, textile, and paper. For this purpose, purification of extracellular protease and amylase produced by the bacterium, Corynebacterium alkanolyticum ATH3 (Acc. No. JX656749) isolated from the distal intestine of a freshwater fish, Anabas testudineus, was carried out using column chromatography. The specific activity of protease and amylase significantly increased with each step of purification and finally became 93.73 and 88.1 U/mg protein with a purification fold 26.03 and 44.94, respectively. The sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed molecular weight of purified protease and amylase was similar to 17 and similar to 28 kDa, respectively. To the authors' knowledge, this is the first report of similar to 17 kDa protease and similar to 28 kDa amylase from the bacterial strain C. alkanolyticum ATH3. Further, enzyme activity was also evidenced by zymography analysis. The enzymes acted optimally at pH 7.5-8.0 and temperature 35-45 degrees C, respectively. So, due to cheapest source, these two enzymes are very important for various purposes in industrial sectors.
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页码:9 / 16
页数:8
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