Influence of insulin treatment on the lacrimal gland and ocular surface of diabetic rats

被引：42

作者：

Modulo, Carolina Maria ^{[1
]}

Jorge, Angelica Gobbi ^{[1
]}

Dias, Ana Carolina ^{[1
]}

Braz, Alexandre Martins ^{[1
]}

Bertazolli-Filho, Rubens ^{[2
]}

Jordao, Alceu A., Jr. ^{[3
]}

Marchini, J. Sergio ^{[3
]}

Rocha, Eduardo M. ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Ophthalmol, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil

[2] Univ Fed Parana, Ctr Politecn, Dept Cell Biol, Biol Sector, BR-81531900 Curitiba, PR, Brazil

[3] Univ Sao Paulo, Dept Clin Med, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil

来源：

ENDOCRINE | 2009年 / 36卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

Diabetes mellitus; Dry eye; Insulin; Lacrimal gland; Oxidative damage; TEAR FILM; SIGNALING PATHWAYS; LIPID-PEROXIDATION; RADICAL FORMATION; SALIVARY-GLANDS; VITAMIN-E; SECRETION; CELLS; RETINOPATHY; GLUTATHIONE;

D O I：

10.1007/s12020-009-9208-9

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Previous studies have observed changes in the lacrimal gland and ocular surface related to diabetes mellitus and related it to insulin resistance or insufficiency and oxidative damage. The aim of this study was to evaluate whether insulin treatment inhibits those changes. Diabetes was induced in male Wistar rats with a single intravenous injection of streptozotocin and a subgroup was treated with insulin. After 5 and 10 weeks, the three groups (n = 5-10/group/experimental procedure) were compared for biochemical, functional, and histological parameters. After 5 weeks, changes in morphology and increased numbers of lipofucsin-like inclusions were observed in lacrimal glands of diabetic but not insulin-treated rats. After 5 weeks, malonaldehyde and total peroxidase activity were significantly higher in diabetic rats, but similar to control in insulin-treated diabetic rats (P = 0.03, P = 0.02, respectively). Our data indicate that diabetes induces histological alterations in lacrimal gland and suggests that hyperglycemia-related oxidative stress may participate in diabetic dry eye syndrome. Prevention by insulin replacement suggests direct hormone action and/or benefit by early sub optimal metabolic control.

引用

页码：161 / 168

页数：8

共 55 条

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