Direct separation and quantitative determination of transglimepiride by high performance liquid chromatography using an amide type chiral stationary phase

Trans- and cis-glimepiride were directly separated by a new established high performance chromatographic method, which involved the use of Chirex 3005 column. Several parameters were studied. Simple and reliable separation was achieved, with the separation factor (alpha) 1.12 and the resolution factor (R-s) 1. 86, when using the optimized mobile phase composed of n-hexane: 1,2-dicholoethane: methanol (88/8/4 by vol) at 25degreesC and 1.8 mL/min. Standard linear calibration curve was established for trans-glimepiride, over the range of 3 similar to 320 mg/L, with the correlation coefficient of 1. 0000. The minimum limit of detection was 0. 47 mg/L. Precision of the assay was gratifying and the method has been used to monitor and identify quantitatively the products of glimepiride.