Expression of a human lactoferrin N-lobe in Nicotiana benthmiana with potato virus X-based agroinfection

被引：27

作者：

Li, Y

Geng, YF

Song, HH

Zheng, GY

Huan, LD

Qiu, BS ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, Mol Microbiol Ctr, Beijing 10080, Peoples R China

[2] Beijing Normal Univ, Coll Life Sci, Beijing 10080, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 12期

关键词：

agroinfection; antimicrobial activity; human lactoferrin; N-lobe; potato virus X;

D O I：

10.1023/B:BILE.0000030038.27358.20

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A DNA fragment encoding the N-terminal half (N-lobe) of the human lactoferrin (hLfN) gene has now been cloned into recombinant Potexvirus potato virus X (PVX) vector and expressed in Nicotiana benthmiana using agroinfection. Western blot analysis showed the recombinant protein with an apparent molecular mass on electrophoresis of ca. 40 kDa, corresponding to the predicted size of the hLfN. The yield of hLfN reached a maximum (up to 0.6% of total soluble proteins) when recombinant PVX systemically infected an entire plant. Protein extracts from infected plants had antibacterial activity.

引用

页码：953 / 957

页数：5

共 12 条

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