Association of XRCC1 and ERCC2 promoters' methylation with chromatin condensation and sperm DNA fragmentation in idiopathic oligoasthenoteratozoospermic men

被引：10
作者：
Metin Mahmutoglu, Asli [1 ]
Gunes, Sezgin [1 ,2 ]
Asci, Ramazan [2 ,3 ]
Henkel, Ralf [4 ,5 ]
Aydin, Oguz [6 ]
机构：
[1] Ondokuz Mayis Univ, Dept Med Biol, Fac Med, TR-55139 Samsun, Turkey
[2] Ondokuz Mayis Univ, Grad Sch Hlth Sci, Dept Multidisciplinary Mol Med, Samsun, Turkey
[3] Ondokuz Mayis Univ, Dept Urol, Fac Med, Samsun, Turkey
[4] Univ Western Cape, Dept Med Biosci, Bellville, South Africa
[5] Cleveland Clin, Amer Ctr Reprod Med, Cleveland, OH 44106 USA
[6] Ondokuz Mayis Univ, Dept Pathol, Fac Med, Samsun, Turkey
来源：
ANDROLOGIA | 2021年 / 53卷 / 02期
关键词：
chromatin condensation; ERCC2; idiopathic male infertility; sperm DNA fragmentation; XRCC1; REPAIR GENE; POLYMORPHISMS; INFERTILITY; AZOOSPERMIA; DAMAGE; RISK; MECHANISMS; EXPRESSION; PARAMETERS; TUNEL;
D O I：
10.1111/and.13925
中图分类号：
R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
摘要：
The aim of the study was to investigate whether the promoter methylation of XRCC1 and ERCC2 genes is associated with sperm DNA fragmentation and chromatin condensation in idiopathic oligoasthenoteratozoospermic men. This study involved 77 infertile men with idiopathic oligoasthenoteratozoospermia and 51 normozoospermic controls. The methylight method, TUNEL assay and aniline blue staining were used for the evaluation of XRCC1 and ERCC2 genes' methylation, SDF and sperm chromatin condensation, respectively. SDF (p = .004) and XRCC1 methylation (p = .0056) were found to be significantly higher in men with idiopathic OAT than in the controls, while mature spermatozoa frequency was higher in controls as compared to infertile men (p < .0001). No significant association was found between SDF and methylation of XRCC1 and ERCC2 genes (p = .9277 and p = .8257, respectively). However, compared to the cut-off point obtained by receiver operating characteristic analysis, a significant association was found between SDF and XRCC1 methylation, positive and negative methylation groups, generated according to the cut-off value for XRCC1. XRCC1 methylation was found to have a significant effect on chromatin condensation (p = .0017). No significant difference was detected among ERCC2 methylation, male infertility and SDF. In conclusion, XRCC1 methylation may have a role in sperm chromatin condensation and idiopathic OAT.
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页数：10
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