Tailoring the surface properties of tobacco mosaic virions by the integration of bacterially expressed mutant coat protein

被引:24
作者
Eiben, Sabine [1 ]
Stitz, Nina [2 ]
Eber, Fabian [1 ]
Wagner, Jerrit [2 ]
Atanasova, Petia [2 ]
Bill, Joachim [2 ]
Wege, Christina [1 ]
Jeske, Holger [1 ]
机构
[1] Univ Stuttgart, Inst Biol, D-70569 Stuttgart, Germany
[2] Univ Stuttgart, Inst Mat Sci, D-70569 Stuttgart, Germany
关键词
TMV; Mineralisation; Self-assembly; In vitro; E; coli; LIGHT-HARVESTING ARRAYS; NUCLEOTIDE-SEQUENCE; VIRUS-PARTICLES; IN-VIVO; TMV; RNA; NANOPARTICLES; MAGNETOVISCOSITY; MINERALIZATION; FERROFLUIDS;
D O I
10.1016/j.virusres.2013.11.019
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Due to its small dimensions and high stability, tobacco mosaic virus (TMV) is used as nano-scaffold frequently. Its surface can be engineered to meet specific needs for technical, medical or materials applications. However, not all technically desirable TMV coat protein (CP) mutants can be propagated in plants successfully, if they change the efficiency of virion assembly. In order to circumvent this problem, a novel wild type (wt) CP-assisted and RNA-directed assembly procedure was designed for a recalcitrant CP mutant: Although pure hexahistidine-tagged CP cannot form particles on its own with TMV RNA in vitro, it was integrated into full-length particles if blended with wt CP in different proportions. The resulting rods formed dense monolayers with short range alignment on silicon substrates, substantially different from the largely wavy patterns obtained with wt TMV. Since they also mediated efficient ZnO deposition under mild conditions, the approach has yielded a new class of biotemplates which are amenable to the formation of nanostructured hybrid materials with adjustable texture for various applications. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:92 / 96
页数:5
相关论文
共 47 条
[1]   The physics of tobacco mosaic virus and virus-based devices in biotechnology [J].
Alonso, J. M. ;
Gorzny, M. L. ;
Bittner, A. M. .
TRENDS IN BIOTECHNOLOGY, 2013, 31 (09) :530-538
[2]   Virus-Templated Synthesis of ZnO Nanostructures and Formation of Field-Effect Transistors [J].
Atanasova, Petia ;
Rothenstein, Dirk ;
Schneider, Joerg J. ;
Hoffmann, Rudolf C. ;
Dilfer, Stefan ;
Eiben, Sabine ;
Wege, Christina ;
Jeske, Holger ;
Bill, Joachim .
ADVANCED MATERIALS, 2011, 23 (42) :4918-4922
[3]   New Approaches for Bottom-Up Assembly of Tobacco Mosaic Virus-Derived Nucleoprotein Tubes on Defined Patterns on Silica- and Polymer-Based Substrates [J].
Azucena, Carlos ;
Eber, Fabian J. ;
Trouillet, Vanessa ;
Hirtz, Michael ;
Heissler, Stefan ;
Franzreb, Matthias ;
Fuchs, Harald ;
Wege, Christina ;
Gliemann, Hartmut .
LANGMUIR, 2012, 28 (42) :14867-14877
[4]   Catalytic coating of virus particles with zinc oxide [J].
Balci, S. ;
Bittner, A. M. ;
Schirra, M. ;
Thonke, K. ;
Sauer, R. ;
Hahn, K. ;
Kadri, A. ;
Wege, C. ;
Jeske, H. ;
Kern, K. .
ELECTROCHIMICA ACTA, 2009, 54 (22) :5149-5154
[5]   Display of epitopes on the surface of tobacco mosaic virus: Impact of charge and isoelectric point of the epitope on virus-host interactions [J].
Bendahmane, M ;
Koo, M ;
Karrer, E ;
Beachy, RN .
JOURNAL OF MOLECULAR BIOLOGY, 1999, 290 (01) :9-20
[6]   Role of Hexahistidine in Directed Nanoassemblies of Tobacco Mosaic Virus Coat Protein [J].
Bruckman, Michael A. ;
Soto, Carissa M. ;
McDowell, Heather ;
Liu, Jinny L. ;
Ratna, Banahalli R. ;
Korpany, Katalin V. ;
Zahr, Omar K. ;
Blum, Amy Szuchmacher .
ACS NANO, 2011, 5 (03) :1606-1616
[7]   ASSEMBLY OF TOBACCO MOSAIC-VIRUS [J].
BUTLER, PJG .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1976, 276 (943) :151-163
[8]   Self-assembly of tobacco mosaic virus: the role of an intermediate aggregate in generating both specificity and speed [J].
Butler, PJG .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1999, 354 (1383) :537-550
[9]  
Caspar D L, 1990, Adv Biophys, V26, P157, DOI 10.1016/0065-227X(90)90011-H
[10]  
CASPAR DLD, 1963, ADV PROTEIN CHEM, V18, P37