The exocyst localizes to the primary cilium in MDCK cells

被引：75
作者：
Rogers, KK
Wilson, PD
Snyder, RW
Zhang, XY
Guo, W
Burrow, CR
Lipschutz, JH [1 ]
机构：
[1] Univ Penn, Dept Med, Philadelphia, PA 19104 USA
[2] CUNY Mt Sinai Sch Med, Dept Med, New York, NY 10029 USA
[3] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA
[4] Univ Penn, Cell & Mol Biol Grad Grp, Philadelphia, PA 19104 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 319卷 / 01期
关键词：
exocyst; cilia; ADPKD;
D O I：
10.1016/j.bbrc.2004.04.165
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Primary cilia play a role in the maintenance of tubular epithelial differentiation and ciliary dysfunction can result in abnormal cyst formation, such as occurs in autosomal dominant polycystic kidney disease (ADPKD). We previously showed that the exocyst, an eight-protein complex involved in the biogenesis of polarity from yeast to mammals, is centrally involved in cyst formation [Mol. Biol. Cell. 11 (2000) 4259]. Here we show that the exocyst complex localizes to the primary cilium in Madin-Darby canine kidney (MDCK) tubular epithelial cells. We further show that the exocyst is overexpressed in both cell lines and primary cell cultures of ADPKD origin, suggesting that the exocyst may be involved in the pathogenesis of ADPKD. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码：138 / 143
页数：6
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