Engineering of Radioiodine-Labeled Gold Core Shell Nanoparticles As Efficient Nuclear Medicine Imaging Agents for Trafficking of Dendritic Cells

被引：47

作者：

Lee, Sang Bong ^{[1
,2
]}

Lee, Sang-Woo ^{[1
,2
]}

Jeong, Shin Young ^{[1
]}

Yoon, GhilSuk ^{[3
]}

Cho, Sung Jin ^{[5
,6
]}

Kim, Sang Kyoon ^{[7
]}

Lee, In-Kyu ^{[4
]}

Ahn, Byeong-Cheol ^{[1
]}

Lee, Jaetae ^{[1
,5
]}

Jeon, Yong Hyun ^{[1
,4
,7
]}

机构：

[1] Kyungpook Natl Univ Hosp, Dept Nucl Med, Daegu, South Korea

[2] Kyungpook Natl Univ Hosp, Leading Edge Res Ctr Drug Discovery & Dev Diabet, Daegu, South Korea

[3] Kyungpook Natl Univ, Dept Pathol, Sch Med, Daegu 41944, South Korea

[4] Kyungpook Natl Univ, Dept Internal Med, Sch Med, Daegu 41944, South Korea

[5] Daegu Gyeongbuk Med Innovat Fdn, Daegu 41061, South Korea

[6] Daegu Gyeongbuk Med Innovat Fdn, New Drug Dev Ctr, Daegu 41061, South Korea

[7] Daegu Gyeongbuk Med Innovat Fdn, Lab Anim Ctr, Daegu 41061, South Korea

来源：

ACS APPLIED MATERIALS & INTERFACES | 2017年 / 9卷 / 10期

基金：

新加坡国家研究基金会;

关键词：

dendritic cells; cell tracking; tannic acid; functionalized gold core-shell nanoparticles; detection limit; PET imaging; POSITRON-EMISSION-TOMOGRAPHY; CANCER-IMMUNOTHERAPY; TRACKING; STABILITY; THERAPY; PROBES; PET;

D O I：

10.1021/acsami.6b14800

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The development of highly sensitive, stable, and biocompatible imaging agents allowing visualization of dendritic cell (DC) migration is one of the essential factors for effective DC-based immunotherapy. Here, we used a novel and efficient synthesis approach to develop radioiodine-124-labeled tannic acid gold core shell nanoparticles(I-124-TA-Au@AuNPs) for DC labeling and in vivo tracking of their migration using positron emission tomography (PET). I-124-TA-Au@AuNPs were produced within 40 min in high yield via straightforward tannic acid-mediated radiolabeling chemistry and incorporation of Au shell, which resulted in high radio-sensitivity and excellent chemical stability of nanoparticles in DCs and living mice. I-124-TA-Au@AuNPs demonstrated good DC labeling efficiency and did not affect cell biological functions, including proliferation and phenotype marker expression. Importantly, I-124-TA-Au@AuNPs in an extremely low amount (0.1 mg/kg) were successfully applied to track the migration of DCs to lymphoid organs (draining lymph nodes) in mice.

引用

页码：8480 / 8489

页数：10