Inhibition of Cronobacter sakazakii Virulence Factors by Citral

被引：76

作者：

Shi, Chao ^{[1
]}

Sun, Yi ^{[1
]}

Liu, Zhiyuan ^{[1
]}

Guo, Du ^{[1
]}

Sun, Huihui ^{[1
]}

Sun, Zheng ^{[1
]}

Chen, Shan ^{[1
]}

Zhang, Wenting ^{[1
]}

Wen, Qiwu ^{[1
]}

Peng, Xiaoli ^{[1
]}

Xia, Xiaodong ^{[1
,2
]}

机构：

[1] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China

[2] Northwest A&F Univ, SinoUS Joint Res Ctr Food Safety, Yangling 712100, Shaanxi, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

中国国家自然科学基金;

关键词：

ENTEROBACTER-SAKAZAKII; BIOFILM FORMATION; ESCHERICHIA-COLI; CHROMOBACTERIUM-VIOLACEUM; LISTERIA-MONOCYTOGENES; STAPHYLOCOCCUS-AUREUS; TRANS-CINNAMALDEHYDE; STAINLESS-STEEL; INFANT FORMULA; ESSENTIAL OILS;

D O I：

10.1038/srep43243

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cronobacter sakazakii is a foodborne pathogen associated with fatal forms of necrotizing enterocolitis, meningitis and sepsis in neonates and infants. The aim of this study was to determine whether citral, a major component of lemongrass oil, could suppress putative virulence factors of C. sakazakii that contribute to infection. Sub-inhibitory concentrations of citral significantly decreased motility, quorum sensing, biofilm formation and endotoxin production. Citral substantially reduced the adhesion and invasion of C. sakazakii to Caco-2 cells and decreased bacterial survival and replication within the RAW 264.7 macrophage cells. Citral also repressed the expression of eighteen genes involved in the virulence. These findings suggest that citral has potential to be developed as an alternative or supplemental agent to mitigate the infections caused by C. sakazakii.

引用

页数：11

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