A Facile Method for Reversibly Linking a Recombinant Protein to DNA

被引：59

作者：

Goodman, Russell P. ^{[1
]}

Erben, Christoph M. ^{[1
]}

Malo, Jonathan ^{[1
]}

Ho, Wei M. ^{[1
]}

McKee, Mireya L. ^{[1
]}

Kapanidis, Achillefs N. ^{[1
]}

Turberfield, Andrew J. ^{[1
]}

机构：

[1] Univ Oxford, Dept Phys, Clarendon Lab, Oxford OX1 3PU, England

来源：

CHEMBIOCHEM | 2009年 / 10卷 / 09期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

DNA; nanostructures; nanotechnology; proteins; self-assembly; RESONANCE ENERGY-TRANSFER; METAL CHELATE ADSORBENT; IMMUNO-PCR; FLUORESCENT PROTEINS; ARRAYS; MOLECULES; STREPTAVIDIN; GREEN; NANOSTRUCTURES; OXIDOREDUCTASE;

D O I：

10.1002/cbic.200900165

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a facile method for linking recombinant proteins to DNA. It is based on the nickel-mediated interaction between a hexahistidine tag (His(6)-tag) and DNA functionalized with three nitrilotriacetic acid (NTA) groups. The resulting DNA-protein linkage is site-specific. It can be broken quickly and controllably by the addition of a chelating agent that binds nickel. We have used this new linker to bind proteins to a variety of DNA motifs commonly used in the fabrication of nanostructures by DNA self-assembly.

引用

页码：1551 / 1557

页数：7

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