A novel bend of DNA CIT: Changeable bending-center sites of an intrinsic curvature under temperature conditions

被引:18
作者
Agrawal, GK [1 ]
Asayama, M [1 ]
Shirai, M [1 ]
机构
[1] IBARAKI UNIV,DIV BIOTECHNOL,MOL MICROBIOL LAB,AMI,IBARAKI 30003,JAPAN
关键词
cyanobacterium; distamycin; Microcystis aeruginosa; psbA gene; static bend; transcription;
D O I
10.1016/S0378-1097(96)00517-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We found a novel DNA curvature, which has changeable bending-center sites of an intrinsic curvature under temperature conditions (CIT) in the cyanobacterium strain Microcystis aeruginosa K-81. Circular permutation analyses (CPA) for CIT under different temperature conditions (4-50 degrees C) revealed that the changeable bending-center sites are located in the 5'-upstream region (-141 to -184) of the psbA2 gene, encoding the Dl protein homolog for photosynthesis. The nucleotide sequence around the bending center contains several dT (deoxy thymine) tracts, which seem to be a pivotal determinant for CIT.
引用
收藏
页码:139 / 145
页数:7
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