The development of marine biomaterial derived from decellularized squid mantle for potential application as tissue engineered urinary conduit

被引：10

作者：

Adamowicz, J. ^{[1
,2
]}

Kloskowski, T. ^{[1
]}

Stopel, M. ^{[3
]}

Gniadek, M. ^{[4
]}

Rasmus, M. ^{[1
]}

Balcerczyk, D. ^{[1
]}

Buhl, M. ^{[1
]}

Gagat, M. ^{[5
]}

Antosik, P. ^{[6
]}

Grzanka, D. ^{[6
]}

Sionkowska, A. ^{[7
]}

Drewa, T. ^{[1
]}

Pokrywczynska, M. ^{[1
]}

机构：

[1] Nicolaus Copernicus Univ, Coll Med, Dept Regenerat Med, Chair Urol, Curie Sklodowskiej 9, PL-85094 Bydgoszcz, Poland

[2] European Assoc Urol, Young Acad Urologists, Reconstruct Urol Working Grp, Arnhem, Netherlands

[3] Univ Sci & Technol, Dept Mech & Comp Methods, Kaliskiego 7, PL-85796 Bydgoszcz, Poland

[4] Univ Sci & Technol, Dept Fundamentals Machine Design & Biomed Engn, Kaliskiego 7, PL-85796 Bydgoszcz, Poland

[5] Nicolaus Copernicus Univ, Coll Med, Dept Histol & Embryol, Bydgoszcz Curie Sklodowskiej 9, PL-85094 Bydgoszcz, Poland

[6] Nicolaus Copernicus Univ, Coll Med, Dept Clin Pathomorphol, Bydgoszcz Curie Sklodowskiej 9, PL-85094 Bydgoszcz, Poland

[7] Nicolaus Copernicus Univ, Dept Chem Biomat & Cosmet, Gagarina 11, PL-87100 Torun, Poland

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2021年 / 119卷

关键词：

Marine biomaterial; Tissue engineering; Urinary conduit; Decellularized biomaterial; Reconstructive urology; ILEAL CONDUIT; BLADDER; LOCOMOTION; MECHANICS; DIVERSION; JET;

D O I：

10.1016/j.msec.2020.111579

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Tissue engineering is focusing research effort on search for new biomaterials that might be applied to create artificial urinary conduit. Nevertheless, the demanding biomechanical characteristics necessary for proper conduit function is difficult to be replicated. In this study, we are introducing novel marine biomaterial obtained by decellularization of squid mantle derived from Loligo vulgaris. Squid mantles underwent decellularization according to developed dynamic flow two-staged procedure. Efficacy of the method was confirmed by computational dynamic flow analysis. Subsequently Decellularized Squid Mantle (DSM) underwent extensive histological analysis and mechanical evaluation. Based on gained biomechanical data the computational modelling using finite element method was utilized to simulate behavior of DSM used as a urinary conduit. Taking into account potential application in reconstructive urology, the DSM was then evaluated as a scaffold for urothelial and smooth muscle cells derived from porcine urinary bladder. Conducted analysis showed that DSM created favorable environment for cells growth. In addition, due to polarized structure and natural external polysaccharide layer, it protected seeded cells from urine.

引用

页数：17

共 31 条

[1] Development of a conductive biocomposite combining graphene and amniotic membrane for replacement of the neuronal network of tissue-engineered urinary bladder [J].