Dual-site lysosome-targeted fluorescent probe for separate detection of endogenous biothiols and SO2 in living cells

被引：46
作者：
Wu, Ming-Yu [1 ]
Wang, Yue [1 ]
Liu, Yan-Hong [2 ]
Yu, Xiao-Qi [2 ]
机构：
[1] Southwest Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R China
[2] Sichuan Univ, Coll Chem, Minist Educ, Key Lab Green Chem & Technol, Chengdu 610064, Peoples R China
来源：
JOURNAL OF MATERIALS CHEMISTRY B | 2018年 / 6卷 / 25期
基金：
中国国家自然科学基金;
关键词：
RATIONAL DESIGN; IN-VITRO; METABOLISM; STRATEGY; THIOLS; H2O2; DYES; PH;
D O I：
10.1039/c8tb01152d
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
Biothiols and SO2 play crucial roles in many physiological and pathological processes. To unravel their complicated interrelationship and cellular cross-talk, it would be highly desirable to develop single-molecule fluorescent probes that can selectively detect biothiols and SO2 via different emission channels. Here, a novel chromenylium derivative, BPO-Py-diNO(2), based on the rational design of dual recognition sites for biothiols and SO2 selectively and sensitively responded to biothiols with near-infrared fluorescence, and to SO2 with green fluorescence. The emission shift for the two channels was 170 nm. BPO-Py-diNO(2) was selectively enriched in lysosomes. It could also be used to evaluate dual-channel imaging of endogenous biothiols and SO2 in living HeLa cells, and it could be used for monitoring the mutual interconversion of biothiols and SO2.
引用
收藏
页码：4232 / 4238
页数：7
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