YAC transgene-mediated olfactory receptor gene choice

被引：0
作者：
Ebrahimi, FAW
Edmondson, J
Rothstein, R
Chess, A
机构：
[1] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA
[2] MIT, Dept Biol, Cambridge, MA USA
[3] Columbia Univ, Coll Phys & Surg, Dept Neurol, New York, NY USA
[4] Columbia Univ, Coll Phys & Surg, Dept Genet & Dev, New York, NY USA
来源：
DEVELOPMENTAL DYNAMICS | 2000年 / 217卷 / 02期
关键词：
transgenic mice; olfactory receptor; olfaction; vomeronasal organ; olfactory epithelium; allelic inactivation; gene expression;
D O I：
10.1002/(SICI)1097-0177(200002)217:2<225::AID-DVDY9>3.0.CO;2-3
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
In the mouse, individual olfactory neurons express one of a thousand distinct olfactory receptor genes. Furthermore, only one allele of the expressed gene is transcribed, This phenomenon, random allelic inactivation, along with the observation that the olfactory receptor genes reside in. large chromosomal arrays, suggests a role for long-range gene regulation in olfactory receptor gene choice, We have constructed a 300-kb yeast artificial chromosome (YAC) transgene in which a single receptor gene is marked whine maintaining its coding region. This 300-kb piece of DNA functions as an independent olfactory receptor gene locus in directing olfactory receptor gene choice in both the olfactory system and the accessory olfactory system (vomeronasal organ, VNO), Furthermore, the transgene, like endogenous olfactory receptor loci, is subject to allelic inactivation, (C) 2000 Wiley-Liss, Inc.
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页码：225 / 231
页数：7
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