Modulation of binding properties of amphiphilic DNA containing multiple dodecyl phosphotriester linkages to lipid bilayer membrane

被引：8

作者：

Makishi, Shingo ^{[1
]}

Shibata, Tomonori ^{[1
]}

Okazaki, Masatsugu ^{[1
]}

Dohno, Chikara ^{[1
,2
]}

Nakatani, Kazuhiko ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Dept Regulatory Bioorgan Chem, Ibaraki 5670047, Japan

[2] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2014年 / 24卷 / 15期

基金：

日本科学技术振兴机构;

关键词：

Amphiphilic DNA; Lipid membrane; Liposome; SPR; NANOCONSTRUCTS; ENABLES; HYBRID; FUSION;

D O I：

10.1016/j.bmcl.2014.05.042

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

DNA is a promising functional molecule to modify and design lipid membrane functions. In order to use DNA in a hydrophilic-hydrophobic interface including lipid membrane, we have developed an amphiphilic DNA having dodecyl phosphotriester linkages (dod-DNA). Herein, we report the binding of a series of amphiphilic dod-DNAs to the lipid bilayer membrane. Surface plasmon resonance (SPR) assay and fluorescent microscopy showed that dod-DNA having three dodecyl groups at each end strongly bound to lipid membrane due to the slow dissociation rate and the dod-DNA can be used as a linear template for molecular arrangement on the membrane surface. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：3578 / 3581

页数：4

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