Modulation of binding properties of amphiphilic DNA containing multiple dodecyl phosphotriester linkages to lipid bilayer membrane

被引：8

作者：

Makishi, Shingo ^{[1
]}

Shibata, Tomonori ^{[1
]}

Okazaki, Masatsugu ^{[1
]}

Dohno, Chikara ^{[1
,2
]}

Nakatani, Kazuhiko ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Dept Regulatory Bioorgan Chem, Ibaraki 5670047, Japan

[2] Japan Sci & Technol Agcy, PRESTO, Kawaguchi, Saitama 3320012, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2014年 / 24卷 / 15期

基金：

日本科学技术振兴机构;

关键词：

Amphiphilic DNA; Lipid membrane; Liposome; SPR; NANOCONSTRUCTS; ENABLES; HYBRID; FUSION;

D O I：

10.1016/j.bmcl.2014.05.042

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

DNA is a promising functional molecule to modify and design lipid membrane functions. In order to use DNA in a hydrophilic-hydrophobic interface including lipid membrane, we have developed an amphiphilic DNA having dodecyl phosphotriester linkages (dod-DNA). Herein, we report the binding of a series of amphiphilic dod-DNAs to the lipid bilayer membrane. Surface plasmon resonance (SPR) assay and fluorescent microscopy showed that dod-DNA having three dodecyl groups at each end strongly bound to lipid membrane due to the slow dissociation rate and the dod-DNA can be used as a linear template for molecular arrangement on the membrane surface. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：3578 / 3581

页数：4

共 9 条

[1] Fluorescence-based binding assay of hydrophobic DNA to the lipid bilayer membrane
Tomonori, Shibata
Shingo, Makishi
Chikara, Dohno
Kazuhiko, Nakatani
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2012, 243
[2] Design and synthesis of PEGylated amphiphilic block oligomers as membrane anchors for stable binding to lipid bilayer membranes
Daiki Takahashi
Yuta Koda
Yoshihiro Sasaki
Kazunari Akiyoshi
Polymer Journal, 2018, 50 : 787 - 797
[3] Design and synthesis of PEGylated amphiphilic block oligomers as membrane anchors for stable binding to lipid bilayer membranes
Takahashi, Daiki
Koda, Yuta
Sasaki, Yoshihiro
Akiyoshi, Kazunari
POLYMER JOURNAL, 2018, 50 (08) : 787 - 797
[4] Deciphering Anesthetic Action of Noble Gases through their Modulation of Membrane Protein and Lipid Bilayer Properties
Murail, Samuel
Pullumbi, Pluton
Baaden, Marc
BIOPHYSICAL JOURNAL, 2017, 112 (03) : 553A - 554A
[5] Lipid Bilayer Properties Control Membrane Partitioning, Binding, and Transport of P-Glycoprotein Substrates
Clay, Adam T.
Sharom, Frances J.
BIOCHEMISTRY, 2013, 52 (02) : 343 - 354
[6] Controlled Co-reconstitution of Multiple Membrane Proteins in Lipid Bilayer Nanodiscs Using DNA as a Scaffold
Raschle, Thomas
Lin, Chenxiang
Jungmann, Ralf
Shih, William M.
Wagner, Gerhard
ACS CHEMICAL BIOLOGY, 2015, 10 (11) : 2448 - 2454
[7] Modulation of the ligand binding properties of the transcription repressor NmrA by GATA-containing DNA and site-directed mutagenesis
Lamb, HK
Ren, JS
Park, A
Johnson, C
Leslie, K
Cocklin, S
Thompson, P
Mee, C
Cooper, A
Stammers, DK
Hawkins, AR
PROTEIN SCIENCE, 2004, 13 (12) : 3127 - 3138
[8] Conformational and lipid bilayer-perturbing properties of Marburg virus GP2 segments containing the fusion loop and membrane-proximal external region/transmembrane domain
Liu, Nina
Girvin, Mark E.
Brenowitz, Michael
Lai, Jonathan R.
HELIYON, 2019, 5 (12)
[9] MULTIPLE INTERACTIONS OF A DNA-BINDING PROTEIN INVIVO .3. PHAGE-T4 GENE-32 MUTATIONS DIFFERENTIALLY AFFECT INSERTION-TYPE RECOMBINATION AND MEMBRANE PROPERTIES
MOSIG, G
BERQUIST, W
BOCK, S
GENETICS, 1977, 86 (01) : 5 - 23

← 1 →