Thin-layer chromatography of bryostatins

被引：0
作者：
Yamashita, A
Kamano, Y
Nogawa, T
Hiraide, H
Kazama, T
Tozawa, M
Herald, CL
Pettit, GR
机构：
[1] Kanagawa Univ, Fac Sci, Hiratsuka, Kanagawa 2591293, Japan
[2] Arizona State Univ, Dept Chem, Tempe, AZ 85287 USA
[3] Arizona State Univ, Canc Res Inst, Tempe, AZ 85287 USA
来源：
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC | 1999年 / 12卷 / 05期
关键词：
thin-layer chromatography; multiple one-dimensional separation; multiple two-dimensional separation; bryostatins; Bugula neritina;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The separation of eleven pure bryostatins by thin-layer chromatography has been accomplished on silica gel plates with n-hexane-acetone, 7 + 3, and n-hexaae-ethyl acetate, 1 + 1, for normal phase chromatography and methanol-water, 3 + 1, for reversed phase chromatography: The technique was suitable for analysis of bryostatins in bioactive fractions from extracts of the marine bryozoan Bugula neritina. The method was also used in support of structure elucidation, the acetylation of bryostatins 1 and 2, and the hydrolysis of acetates; the hydrolysis of bryostatins 4-11 was also studied by TLC.
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收藏
页码：336 / 339
页数：4
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